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Volume 18, Number 9—September 2012

Letter

Aeromonas spp. Bacteremia in Pregnant Women, Thailand–Myanmar Border, 2011

Suggested citation for this article

To the Editor: Aeromonas spp. bacteria cause a broad spectrum of human infections (1). Invasive infections can be associated with exposure to contaminated water and occur frequently in patients with underlying immunosuppression or trauma but only infrequently in pregnant women (2). During January–May 2011, Aeromonas spp. bacteremia was identified in 3 pregnant Myanmar women of Karen ethnicity, who sought care at migrant/refugee clinics on the Thailand–Myanmar border.

In January 2011, a 31-year-old woman in week 12 of pregnancy sought care at the Wang Pha migrant clinic. She reported a brief history of fever, headache, abdominal pain, and vaginal bleeding. She was tachycardic and tachypneic. A complete blood count indicated mild thrombocytopenia (104 × 103/μL). Ampicillin, gentamicin, and metronidazole were empirically prescribed. Ultrasonography confirmed a nonviable fetus, and products of conception were surgically evacuated. Four days later, treatment was changed to oral amoxicillin and ciprofloxacin because of clinical improvement and isolation of A. hydrophila from blood collected at admission. The patient recovered and was discharged after 6 days.

In March 2011, a 25-year-old primigravid woman in week 12 of pregnancy sought care at the Maela refugee camp; she had a 24-hour history of fever, chills, vomiting, diarrhea, and vaginal bleeding. She had recently sought treatment from a traditional birth attendant, who reportedly had inserted 3 sticks into the cervix and performed uterine massage. At admission, the patient was febrile and in shock. Bimanual examination revealed an open cervix with malodorous discharge. Ultrasonography revealed only products of conception. The patient received intravenous fluids and was empirically treated with ampicillin, gentamicin, and metronidazole. After surgical evacuation of the products of conception, the patient’s condition continued to deteriorate. Despite referral to the local hospital, the woman died the next day with clinical features suggestive of septic shock and disseminated intravascular coagulopathy. Complete blood count results were unavailable. A. veronii biovar sobria was isolated from blood collected at admission.

In May 2011, a 50-year-old woman in week 12 of pregnancy was admitted to the Wang Pha Clinic with a history of vaginal bleeding. She was febrile and in shock. Vaginal examination detected an open cervix with malodorous discharge. Complete blood count indicated reference level leukocytes and mild thrombocytopenia (116 × 103 cells/µL). Blood was collected for culture. The patient received intravenous fluids and was empirically treated with ampicillin, gentamicin, and metronidazole. Products of conception were removed under ultrasonographic guidance. Three days later, treatment was changed to ceftriaxone and metronidazole because of continuing fever and positive blood culture findings (gram-positive cocci and gram-negative bacilli). These organisms were subsequently identified as Streptococcus pyogenes and A. veronii biovar sobria. The patient’s condition improved rapidly, and after 5 days she was discharged with prescriptions for oral amoxicillin and ciprofloxacin.

Blood culture isolates were provisionally identified as Aeromonas spp. by Gram stain, oxidase test, and API 20NE and 20E kits (bioMérieux, Marcy L’Étoile, France). The Aerokey II algorithm was used to identify the isolates to the species level (Table) (3). Susceptibilities to antimicrobial drugs were obtained by the agar disk–diffusion method, according to Clinical and Laboratory Standards Institute guidelines (4).

One other case of invasive Aeromonas spp. infection during pregnancy (A. hydrophila sepsis at week 24 of gestation) has been reported; the focus of infection in that case was hepatobiliary, and normal pregnancy outcome was documented (2). For the 3 case-patients reported here, miscarriage occurred for all. Although products of conception were not submitted for culture, septic abortion was suspected clinically. Of note, A. veronii biovar sobria has been associated with abortion in water buffaloes (5). Nonmedical abortion, including the use of sticks to terminate pregnancy, is reportedly conducted on the Thailand–Myanmar border (6), and its use in 1 of the case-patients reported here was confirmed. Such practices predispose women to infectious complications as a result of the use of nonsterile instruments. Aeromonas spp. can colonize the human genital tract (7). Therefore, the organisms could have gained entry on contaminated abortion sticks or, if the vagina was colonized, as a result of the trauma of the procedure. An alternative hypothesis to explain our clinical findings is that fetal loss occurred as a result of Aeromonas spp. sepsis originating from another exposure.

The isolates were susceptible to a variety of antimicrobial drugs (Table). Empirically prescribed antimicrobial drug protocols for treatment of sepsis during pregnancy (e.g., ampicillin, gentamicin, and metronidazole or an extended-spectrum cephalosporin and metronidazole) should be effective against Aeromonas spp. However, if Aeromonas spp. are isolated, targeted treatment with a fluoroquinolone, extended-spectrum cephalosporin, or carbapenem is more appropriate (1).

We speculate that contamination of sticks used to induce abortion might play a role in these infections in this setting, although obtaining a definitive history to substantiate this speculation is difficult. Also, the scarcity of diagnostic microbiology laboratories in resource-poor settings results in underidentification of the causative pathogens.

Paul TurnerComments to Author , Charlotte Willemse, Kawalee Phakaudom, Thet Wai Zin, Francois Nosten, and Rose McGready
Author affiliations: Shoklo Malaria Research Unit, Mae Sot, Thailand (P. Turner, C. Willemse, K. Phakaudom, T.W. Zin, F. Nosten, R. McGready); Mahidol-Oxford Tropical Medicine Research Unit, Bangkok, Thailand (P. Turner, F. Nosten, R. McGready); and University of Oxford, Oxford, UK (P. Turner, F. Nosten, R. McGready)

Acknowledgment

The Shoklo Malaria Research Unit is part of the Mahidol-Oxford University Tropical Medicine Research Unit, supported by the Wellcome Trust of Great Britain.

References

  1. Janda JM, Abbott SL. The genus Aeromonas: taxonomy, pathogenicity, and infection. Clin Microbiol Rev. 2010;23:3573. DOIPubMed
  2. Damián RF, Zesati RV, Fernaindez LS, Garcia JL. Septicemia due to Aeromonas hydrophila in a pregnant woman: a case report and review of the literature. Infect Dis Obstet Gynecol. 1995;3:2525. DOIPubMed
  3. Carnahan AM, Behram S, Joseph SW. Aerokey II: a flexible key for identifying clinical Aeromonas species. J Clin Microbiol. 1991;29:28439.PubMed
  4. Clinical and Laboratory Standards Institute. Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria, approved guideline. CLSI document M45–A2. Wayne (PA): The Institute; 2010.
  5. Das AM, Paranjape VL. Aeromonas sobria in bubaline (water buffalo) abortion: growth requirement, biochemical characters, antibiotic susceptibility, experimental pathogenicity and serology. Indian J Exp Biol. 1990;28:3415.PubMed
  6. Belton S, Whittaker A. Kathy Pan, sticks and pummelling: techniques used to induce abortion by Burmese women on the Thai border. Soc Sci Med. 2007;65:151223. DOIPubMed
  7. Ekwempu CC, Lawande RV, Egler LJ. Microbial flora of the lower genital tract of women in labour in Zaria, Nigeria. J Clin Pathol. 1981;34:823. DOIPubMed

Table

Suggested citation for this article: Turner P, Willemse C, Phakaudom K, Zin TW, Nosten F, McGready R. Aeromonas spp. bacteremia in pregnant women, Thailand–Myanmar border, 2011 [letter]. Infect Dis [serial on the Internet]. 2012 Sep [date cited]. http://dx.doi.org/10.3201/eid1809.111592

DOI: 10.3201/eid1809.111592

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Table of Contents – Volume 18, Number 9—September 2012

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Paul Turner, Shoklo Malaria Research Unit, PO Box 46, 68/30 Ban Toong Rd, Mae Sot, Thailand 63110





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