We performed a retrospective cohort study of all Ontario residents who had pulmonary nontuberculous Mycobacterium spp. isolated during 1998–2010, identified from the records of the Public Health Ontario Laboratory, capturing ≈95% of NTM disease in Ontario. Culture was performed by using Bactec 460 TB system until 2000 and thereafter with BACTEC MGIT 960 (Becton Dickinson, Baltimore, MD, USA). Before 2008, speciation was performed by using a combination of DNA probes (AccuProbe, Gen-Probe, San Diego, CA, USA) for Mycobacterium avium complex (MAC) and M. gordonae and high-performance liquid chromatography for other species and thereafter solely by DNA probes (AccuProbe, Gen-Probe) or line-probe assays (GenoType, Hain Lifescience, Germany). Because MAC was not identified to individual species for most of our study, we present data only for MAC.

Full criteria for pNTM disease include the presence of all clinical (symptoms and radiology) and microbiological components (1). We defined surrogate criteria as microbiological criteria only (1), (>2 positive sputum cultures or 1 bronchoscopic or lung biopsy culture), which has a positive predictive value of 70%–100% (5,6,9,10). Period prevalence of disease was calculated as the number of persons who fulfilled the disease criteria during a 5-year period (1998–2002 or 2006–2010), divided by the Ontario population at the period midpoint. We left a 3-year gap (2003–2005) between periods to minimize patient overlap. We excluded M. gordonae from period prevalence because it is rarely pathogenic (1). We selected a conservative 5-year period on the assumption that the median survival with pNTM disease is 5–10 years (10,11), using the low end of the survival range based on assumptions that a small proportion of the cohort would not have true disease (misclassified by surrogate definition) and disease of an additional small proportion would be cured.

Annual isolation prevalence (number of persons in a calendar year with >1 pulmonary Mycobacterium spp. isolate divided by the contemporary population) and annual disease prevalence (number of persons in a calendar year whose illnesses fulfilled criteria for disease divided by the contemporary population) are presented for illustrative purposes. A generalized linear model with negative binomial distribution was used to assess annual rate changes, and a simple model binomial approach was used to compare 5-year period prevalence rates by using SAS 9.2 (SAS Institute, Cary, NC, USA). This study was approved by the University of Toronto Research Ethics Board with the requirement for informed consent waived.

Figure

Figure. . . Annual isolation prevalence and disease prevalence per 100,000 persons of pulmonary nontuberculous mycobacteria, Ontario, Canada, 1998–2010.

Ontario’s population increased from 11.3 million to 13.2 million during 1998–2010. Total annual isolations of pulmonary Mycobacterium spp. rose from 11.4 to 22.2 per 100,000 persons (p = 0.0025, mean annual increase 6.3%) (Table 1; Figure). The relative frequency of different nontuberculous Mycobacterium isolates remained constant. The most common pulmonary nontuberculous Mycobacterum isolates in 2010 were MAC (12.2 isolations/100,000 persons), M. xenopi (3.9/100,000), M. gordonae (3.0/100,000), M. fortuitum (0.8/100,000), and M. abscessus (0.6/100,000). Among patients with different Mycobacterium spp. isolates in 2010, the following proportions were judged to have disease: MAC, 52%; M. abscessus, 50%; M. xenopi, 38%; and other non–M. gordonae species, 38%. Annual prevalence for all NTM disease combined rose from 4.9 cases to 9.8 cases per 100,000 persons (p<0.0001, mean annual increase 6.5%) (Table 1; Figure). Five-year prevalence of pNTM disease (M. gordonae excluded) increased from 29.3 cases per 100,000 persons in 1998–2002 to 41.3 per 100,000 in 2006–2010 (p<0.0001) (Table 2).

The 5-year prevalence of pNTM disease was substantial and increased significantly during our population-based assessment in Ontario, Canada. Our measurements of period prevalence (29.3 and 41.3 cases/100,000 persons) were substantially higher than observed in Oregon (8.6/100.000), probably partially because of the shorter period (2 years) and more stringent definition for disease (medical records review) used in the Oregon study (5). Other studies did not present period prevalence for the entire study populations, only by age strata, and used durations of 3 years (6,7) or <11 years (7). We selected a 5-year period assuming it would provide the most accurate estimate of disease prevalence based on the chronic nature of pNTM disease. Prior studies provided age-stratified data, with high period prevalence in older patients (20.4/100,000 to >200/100,000, depending on period length and specific age range) (5–7), as expected, because pNTM disease is a disease of the elderly (1,4,6,12). Although age data were unavailable for our study, annual disease prevalence of pulmonary MAC in Ontario has a strong age association, with an average increase of 14/100,000 per decade increase during 50–80 years (4).

Changes in microbiological methods and the number of samples submitted annually did not account for the increases in pulmonary nontuberculous Mycobacterium isolation (13). The attenuation in the rate of increase in isolation prevalence around the middle of the study corresponded with a previously reported plateau in the annual number of specimens submitted (13). However, the annual isolation prevalence continued to rise, and the annual disease prevalence rose steadily throughout the study period. We suspect a multifactorial explanation for the increase in pNTM disease: an increase in susceptible hosts (aging, chronic lung disease) contributes (4); decades-old increases in water aerosol exposure could cause recent increases in pNTM disease, given the potential latency of pNTM disease; more computed tomographic scanning probably leads to sampling patients with previously unidentified abnormalities; and reduced tuberculosis, with an associated reduction in cross-immunity, may play a role. The latter is supported by observations of increased extrapulmonary NTM infection in children not vaccinated with M. bovis BCG (14,15). pNTM disease in Ontario is substantial and increased greatly from early (1998–2002) to recent (2006–2010) periods.