Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 19, Number 3—March 2013
Research

Lack of Norovirus Replication and Histo-Blood Group Antigen Expression in 3-Dimensional Intestinal Epithelial Cells

Melissa M. Herbst-KralovetzComments to Author , Andrea L. Radtke, Margarita K. Lay, Brooke E. Hjelm, Alice N. Bolick, Shameema S. Sarker, Robert L. Atmar, David H. Kingsley, Charles J. Arntzen, Mary K. Estes, and Cheryl A. Nickerson
Author affiliations: Author affiliations: University of Arizona, Phoenix, Arizona, USA (M.M. Herbst-Kralovetz, A.L. Radtke); Arizona State University, Tempe, Arizona (M.M. Herbst-Kralovetz, A.L. Radtke, B.E. Hjelm, A.N. Bolick, S.S. Sarker, C.J. Arntzen, C.A. Nickerson); Baylor College of Medicine, Houston, Texas, USA (M.K. Lay, R.L. Atmar, M.K. Estes); Pontificia Universidad, Catolica de Chile, Santiago, Chile (M.K. Lay); Translational Genomics Research Institute, Phoenix (B.E. Hjelm); US Department of Agriculture, Dover, Delaware (D.H. Kingsley)

Main Article

Figure 2

′No evidence of productive Norwalk virus (NV) replication in 3-dimensional intestinal aggregates by quantitative reverse transcription PCR analysis. Supernatants (sups) and cell pellets were harvested for RNA at 0, 6, 24, 48, 72, and 96 hours postinoculation (hpi) with live and inactivated NV and analyzed by quantitative reverse transcription PCR. There was no significant increase (p>0.05) in NV RNA copy number over time in the supernatants or cell pellets relative to input virus.

Figure 2. . . No evidence of productive Norwalk virus (NV) replication in 3-dimensional intestinal aggregates by quantitative reverse transcription PCR analysis. Supernatants (sups) and cell pellets were harvested for RNA at 0, 6, 24, 48, 72, and 96 hours postinoculation (hpi) with live and inactivated NV and analyzed by quantitative reverse transcription PCR. There was no significant increase (p>0.05) in NV RNA copy number over time in the supernatants or cell pellets relative to input virus. Error bars indicate SEM.

Main Article

Page created: February 12, 2013
Page updated: February 12, 2013
Page reviewed: February 12, 2013
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external