Emerging Infectious Disease ISSN: 1080-6059

Lack of Norovirus Replication and Histo-Blood Group Antigen Expression in 3-Dimensional Intestinal Epithelial Cells

Melissa M. Herbst-Kralovetz

, Andrea L. Radtke, Margarita K. Lay, Brooke E. Hjelm, Alice N. Bolick, Shameema S. Sarker, Robert L. Atmar, David H. Kingsley, Charles J. Arntzen, Mary K. Estes, and Cheryl A. Nickerson

Author affiliations: Author affiliations: University of Arizona, Phoenix, Arizona, USA (M.M. Herbst-Kralovetz, A.L. Radtke); Arizona State University, Tempe, Arizona (M.M. Herbst-Kralovetz, A.L. Radtke, B.E. Hjelm, A.N. Bolick, S.S. Sarker, C.J. Arntzen, C.A. Nickerson); Baylor College of Medicine, Houston, Texas, USA (M.K. Lay, R.L. Atmar, M.K. Estes); Pontificia Universidad, Catolica de Chile, Santiago, Chile (M.K. Lay); Translational Genomics Research Institute, Phoenix (B.E. Hjelm); US Department of Agriculture, Dover, Delaware (D.H. Kingsley)

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Figure 2

′No evidence of productive Norwalk virus (NV) replication in 3-dimensional intestinal aggregates by quantitative reverse transcription PCR analysis. Supernatants (sups) and cell pellets were harvested for RNA at 0, 6, 24, 48, 72, and 96 hours postinoculation (hpi) with live and inactivated NV and analyzed by quantitative reverse transcription PCR. There was no significant increase (p>0.05) in NV RNA copy number over time in the supernatants or cell pellets relative to input virus.

Figure 2. . . No evidence of productive Norwalk virus (NV) replication in 3-dimensional intestinal aggregates by quantitative reverse transcription PCR analysis. Supernatants (sups) and cell pellets were harvested for RNA at 0, 6, 24, 48, 72, and 96 hours postinoculation (hpi) with live and inactivated NV and analyzed by quantitative reverse transcription PCR. There was no significant increase (p>0.05) in NV RNA copy number over time in the supernatants or cell pellets relative to input virus. Error bars indicate SEM.

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