Zichun Xiang, Zhengde Xie, Zhong Wang, Lili Ren, Yan Xiao, Linlin Li, Guy Vernet, Gláucia Paranhos-Baccalà, Kunling Shen, and Jianwei Wang
Author affiliations: Ministry of Health Key Laboratory of Systems Biology of Pathogens, Beijing, People’s Republic of China (Z. Xiang, L. Ren, L. Li, J. Wang); Institute of Pathogen Biology, Beijing (Z. Xiang, L. Ren, Y. Xiao, L. Li, J. Wang); Beijing Children’s Hospital, Beijing (Z. Xie, K. Shen); Peking Union Medical College Hospital, Beijing (Z. Wang); Fondation Mérieux, Lyon, France (G. Vernet, G. Paranhos-Baccalà)
Figure. . . Phylogenetic tree of human enteroviruses (EVs) for nucleotide sequences of the viral protein (VP) 4/VP2 gene region (435 nt, corresponding to nt positions 654–1,088 of EV-C104 prototype strain CL-12310945 [EU840733]), People’s Republic of China, March 2007–February 2012. The tree was generated with 1,000 bootstrap replicates. Neighbor-joining analysis of targeted nucleotide sequence was performed by using the Kimura 2-parameter model with the Molecular Evolutionary Genetics Analysis software version 4.0 (www.megasoftware.net/). Each strain detected in this study is indicated by a black circle and a specific identification code (BCH/PUMCH), followed by the patient number. Enterovirus 68, cocksackievirus (CV) A2, and echovirus (E) 3 (GenBank accession nos. AY426531, AY421760, and AY302553) were used as outgroups. PV, poliovirus. EV-C denotes the EV species to which EV-C104 belongs. Scale bar indicates evolutionary distance.
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