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Volume 20, Number 8—August 2014
Dispatch

Isolation of MERS Coronavirus from a Dromedary Camel, Qatar, 2014

V. Stalin Raj1, Elmoubasher A.B.A. Farag1, Chantal B.E.M. Reusken, Mart M. Lamers, Suzan D. Pas, Jolanda Voermans, Saskia L. Smits, Albert D.M.E. Osterhaus, Naema Al-Mawlawi, Hamad E. Al-Romaihi, Mohd M. AlHajriComments to Author , Ahmed M. El-Sayed, Khaled A. Mohran, Hazem Ghobashy, Farhoud Alhajri, Mohamed Al-Thani, Salih A. Al-Marri, Mamdouh M. El-Maghraby, Marion P.G. Koopmans, and Bart L. HaagmansComments to Author 
Author affiliations: Erasmus Medical Center, Rotterdam, the Netherlands (V. Stalin Raj, C.B.E.M. Reusken, M.M. Lamers, S.D. Pas, J. Voermans, S.L. Smits, A.D.M.E. Osterhaus, M.P.G. Koopmans, B.L. Haagmans); Supreme Council of Health, Doha , Qatar (E.A.B.A. Farag, H.E. Al-Romaihi, M.M. AlHajri, A.M. El-Sayed, M. Al-Thani, S.A. Al-Marri); Hamad Medical Corporation, Doha (N. Al-Mawlawi); Ministry of Environment, Doha (K.A. Mohran, H. Ghobashy, F. Alhajri, M.M. El-Maghraby)

Main Article

Figure 2

Middle East respiratory syndrome coronavirus (MERS-CoV) from camel replicates in human hepatoma (Huh-7) cells and uses human DPP4 as entry receptor. Huh-7 cells were inoculated with camel MERS-CoV and left for 1 h. Next, cells were washed twice, and supernatant was collected at 2 h (open bars) and 20 h (closed bars) before being tested for MERS-CoV RNA by using a TaqMan assay. We analyzed control camel MERS-CoV–infected cells, cells inoculated with camel MERS-CoV in the presence of normal camel

Figure 2. Middle East respiratory syndrome coronavirus (MERS-CoV) from camel replicates in human hepatoma (Huh-7) cells and uses human DPP4 as entry receptorHuh-7 cells were inoculated with camel MERS-CoV and left for 1 hNext, cells were washed twice, and supernatant was collected at 2 h (open bars) and 20 h (closed bars) before being tested for MERS-CoV RNA by using a TaqMan assayWe analyzed control camel MERS-CoV–infected cells, cells inoculated with camel MERS-CoV in the presence of normal camel serum (NCS), MERS-CoV–antibody positive camel serum (Ab-positive CS), normal goat serum (NGS), and anti-DPP4 polyclonal antibody–treated cellsResults are expressed as genome equivalents (GE), 50% tissue culture infective dose (TCID50/mL) (A)MDCK cells transfected with plasmid-encoding human DPP4 or a control plasmid (pcDNA) were stained with polyclonal antibody against human DPP4 (B) or inoculated with camel MERS-CoV and fixed 20 h after inoculation (p.i.) and stained for viral antigen (C).

Main Article

1These authors contributed equally to this article.

Page created: July 18, 2014
Page updated: July 18, 2014
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