Polycystic Kidney Disease: An Unrecognized Emerging Infectious Disease?
Marcia A. Miller-Hjelle*, J. Thomas Hjelle*, Monica Jones*, William R. Mayberry†, Mary Ann Dombrink-Kurtzman‡, Stephen W. Peterson‡, Deborah M. Nowak*, and Frank S. Darras*
Author affiliations: *University of Illinois College of Medicine at Peoria, Peoria, Illinois, USA; †East Tennessee State University, Johnson City, Tennessee, USA; ‡U.S. Department of Agriculture, Agricultural Research Service, Peoria, Illinois, USA
Figure 2. Amplification results of normal and PKD kidney tissue and cyst fluids with universal fungal primers ITS 1 and NL 4. 2A: DNA from healthy human kidney tissue diluted 1:10, 1:100 and 1:1,000 (lanes 1-3); control fungal DNA, A. tamarii (lane 4); negative control (lane 5); 1 kb ladder (lane 6); arrow indicates migration front. 2B (NL 4) and 2C (ITS 1): two cyst fluids, donor 6, negative for detectable endotoxin and ß-DG (lanes 1 and 5); two cyst fluids, donor 4, positive for ß-DG (lanes 2 and 3) and positive for Fusarium solani antigen (lane 3); two cyst fluids, donor 5, positive for ß-DG (lanes 4 and 6); two PKD kidney tissues, donor 5 (lane 7) and donor 4 (lane 8); negative control (lane 9). Large arrows in 2B and 2C point to 560 bp molecular weight marker; small arrows point to product bands.
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