Volume 3, Number 2—June 1997
From the 1st International Conference on Emerging Zoonoses
From the 1st International Conference on Emerging Zoonoses
Brucellosis: an Overview
Brucellosis remains a major zoonosis worldwide. Although many countries have eradicated Brucella abortus from cattle, in some areas Brucella melitensis has emerged as a cause of infection in this species as well as in sheep and goats. Despite vaccination campaigns with the Rev 1 strain, B. melitensis remains the principal cause of human brucellosis. Brucella suis is also emerging as an agent of infection in cattle, thus extending its opportunities to infect humans. The recent isolation of distinctive strains of Brucella from marine mammals has extended its ecologic range. Molecular genetic studies have demonstrated the phylogenetic affiliation to Agrobacterium, Phyllobacterium, Ochrobactrum, and Rhizobium. Polymerase chain reaction and gene probe development may provide more effective typing methods. Pathogenicity is related to production of lipopolysaccharides containing a poly N-formyl perosamine O chain, Cu-Zn superoxide dismutase, erythrulose phosphate dehydrogenase, stress-induced proteins related to intracellular survival, and adenine and guanine monophosphate inhibitors of phagocyte functions. Protective immunity is conferred by antibody to lipopolysaccharide and T-cell-mediated macrophage activation triggered by protein antigens. Diagnosis still centers on isolation of the organism and serologic test results, especially enzyme immunoassay, which is replacing other methods. Polymerase chain reaction is also under evaluation. Therapy is based on tetracyclines with or without rifampicin, aminoglycosides, or quinolones. No satisfactory vaccines against human brucellosis are available, although attenuated purE mutants appear promising.
Brucellosis has been an emerging disease since the discovery of Brucella melitensis by Bruce in 1887. Subsequently, an increasingly complex pattern of strains has emerged with the identification of Brucella abortus, Brucella suis, Brucella neotomae, Brucella ovis, Brucella canis, and, more recently, types infecting marine mammals. Because each type has distinctive epidemiologic features, with each new type, the complexity of the interaction with humans has increased. Because new strains may emerge and existing types adapt to changing social and agricultural practices, the picture remains incomplete.
This synopsis reviews major advances in the knowledge of certain aspects—genetics, antigenic structure, mechanisms of pathogenicity, diagnosis, treatment, and prevention of the disease—of the Brucella genus and its host interactions.
Worldwide, brucellosis remains a major source of disease in humans and domesticated animals. Although reported incidence and prevalence of the disease vary widely from country to country, bovine brucellosis caused mainly by B. abortus is still the most widespread form (Tables 1-5). In humans, ovine/caprine brucellosis caused by B. melitensis is by far the most important clinically apparent disease. The disease has a limited geographic distribution, but remains a major problem in the Mediterranean region, western Asia, and parts of Africa and Latin America. Recent reemergence in Malta and Oman indicates the difficulty of eradicating this infection (1). Sheep and goats and their products remain the main source of infection, but B. melitensis in cattle has emerged as an important problem in some southern European countries, Israel, Kuwait, and Saudi Arabia. B. melitensis infection is particularly problematic because B. abortus vaccines do not protect effectively against B. melitensis infection; the B. melitensis Rev.1. vaccine has not been fully evaluated for use in cattle. Thus, bovine B. melitensis infection is emerging as an increasingly serious public health problem in some countries. A related problem has been noted in some South American countries, particularly Brazil and Colombia, where B. suis biovar 1 has become established in cattle (2). In some areas, cattle are now more important than pigs as a source of human infection.
The true incidence of human brucellosis is unknown. Reported incidence in endemic-disease areas varies widely, from <0.01 to >200 per 100,000 population (3). While some areas, such as Peru, Kuwait, and parts of Saudi Arabia, have a very high incidence of acute infections, the low incidence reported in other known brucellosis-endemic areas may reflect low levels of surveillance and reporting, although other factors such as methods of food preparation, heat treatment of dairy products, and direct contact with animals also influence risk to the population.
Consumption of contaminated foods and occupational contact remain the major sources of infection. Examples of human-to-human transmission by tissue transplantation or sexual contact are occasionally reported but are insignificant (4). Prevention of human brucellosis depends on the control of the disease in animals. The greatest success has been achieved in eradicating the bovine disease, mainly in industrialized countries (Table 6); however, most countries have control programs. B. melitensis infection has proved more intractable, and success has been limited (Table 7).
Although few recent outbreaks of disease caused by B. suis biovar 4 have been reported (5), foci of the infection persist in the Arctic regions of North America and Russia and constitute a potential hazard for the local population. B. ovis has not been demonstrated to cause overt disease in humans, although it is widespread in sheep (Tables 1-5). B. canis can cause disease in humans, although this is rare even in countries where the infection is common in dogs (6). Precise information on prevalence is lacking, but B. canis has been recorded in the United States, Mexico, Argentina, Spain, China, Japan, Tunisia, and other countries. The recent isolation of distinctive Brucella strains, tentatively named Brucella maris, from marine animals in the United Kingdom and the United States extends the ecologic range of the genus and, potentially, its scope as a zoonosis (7,8). A hitherto unreported incident of laboratory-acquired infection suggests that this type is pathogenic for humans. Infection could result from occupational contact with infected seals or cetaceans.
Characterization of the molecular genetics of Brucella has taken place almost entirely within the past 10 years. The average molecular complexity of the genome is 2.37 x 109 daltons and the molar G + C 58-59% (9). The genus itself is highly homogeneous with all members showing >95% homology in DNA-DNA pairing studies, thus classifying Brucella as a monospecific genus (10). However, the nomenclature proposed by Verger and colleagues, in which all types would be regarded as biovars of B. melitensis, has not been generally adopted on practical grounds. For this reason, although its shortcomings are well known, the old nomenclature has been retained with the former species' names B. abortus, B. melitensis, B. suis, Brucella neotomae, B. ovis, and B. canis being used for the corresponding nomen species (11,12). Within these, seven biovars are recognized for B. abortus (1,7-10,12,13), three for B. melitensis (1, 7,8), and five for B. suis (1,7-10,12). The other species have not been differentiated into biovars, although variants exist (14). The current biotyping system does not encompass all known variants even of the principal species. Thus, variants of B. melitensis have been described; this suggests that the scheme should be extended (11,13,15). The strains isolated from marine animals clearly form a separate group and have been unofficially designated B. maris (E. S. Broughton, unpub. data). At least two subdivisions of this strain can be distinguished, corresponding approximately to strains isolated from cetaceans and seals, respectively (7,8).
Restriction fragment patterns produced by infrequently cutting endonucleases provide support for the current differentiation of the nomen species (16). Restriction endonuclease analysis has generally been unsuccessful for typing when applied to the whole genome (17) but polymerase chain amplification of selected sequences followed by restriction analysis has provided evidence of polymorphism in a number of genes including omp 2, dnaK, htr, and ery (the erythrulose-1-phosphate dehydrogenase gene) (18-20). The omp2 gene is taxonomically important because it determines dye sensitivity, one of the traditional typing methods for biovar differentiation (21). Its polymorphism and capacity for posttranslational modification of its product may explain the tendency for variation in dye sensitivity patterns and have been used as the basis for a genetic classification of Brucella (22,23). The dnaK gene of B. melitensis is cleaved into two fragments by Eco RV endonuclease, whereas the genes of the other nomen species all produce a single fragment (24). The ery gene is reported to have undergone a 7.2 kbp deletion in B. abortus strain 19 (20). This could explain this strain's erythritol sensitivity, a major factor in its attenuation.
The genome of Brucella contains two chromosomes of 2.1 and 1.5 mbp, respectively. Both replicons encode essential metabolic and replicative functions and hence are chromosomes and not plasmids (25,26). Natural plasmids have not been detected in Brucella, although transformation has been effected by wide host range plasmids after conjugative transfer or electroporation (27).
rRNA sequencing has defined the phylogenetic relationship of Brucella. Its closest known relation, Ochrobactrum anthropi, is an environmental bacterium associated with opportunistic infections (28); this organism is also detected by a polymerase chain reaction (PCR) procedure that is otherwise specific for Brucella (29). Possibly more closely related is the incompletely characterized Vibrio cyclosites, which displays >90% similarity of 5S rRNA sequence (30). Less closely related but within the same subgroup of the -2 Proteobacteria are Agrobacterium, Phyllobacterium, and Rhizobium, which also possess multiple replicons and a capacity for intracellular growth. The Bartonella group also shows some affinity to Brucella on the basis of rRNA, but not DNA, similarity (31). Other similarities have been noted in cell membrane lipid composition and intracellular growth.
A substantial number of antigenic components of Brucella have been characterized. However, the antigen that dominates the antibody response is the lipopolysaccharide (LPS). In smooth phase strains (S), the S-LPS comprises a lipid A (containing two types of aminoglycose); distinctive fatty acids (excluding ß-hydroxymyristic acid); a core region containing glucose, mannose, and quinovosamine; and an O chain comprising a homopolymer of approximately 100 residues of 4-formamido-4,6-dideoxymannose (linked predominantly α-1,2 in A epitope-dominant strains with every fifth residue linked -1,3 in M dominant strains) (32).
The difference in linkage influences the shape of the LPS epitopes. The A-dominant type is rod-shaped and is determined by five consecutive α-1,2 linked residues, whereas the M-dominant type is kinked and determined by four residues, including one linked α-1,3 (33). Strains that react with antisera to both A and M epitopes produce LPS of both types in approximately equal proportions (30), consistent with the original hypothesis of Wilson and Miles (34). The presence of 4-amino, 4,6 dideoxymannose in the LPS is also responsible for the antigenic cross-reactivity with Escherichia hermanni and Escherichia coli O:157, Salmonella O:30, Stenotrophomonas maltophilia, Vibrio cholerae O:1, and Yersinia enterocolitica O:9 LPS (32). The structure of the LPS of nonsmooth strains (R-LPS) is basically similar to that of the S-LPS except that the O-chain is either absent or reduced to a few residues. The specificity of the R-LPS is, therefore, largely determined by the core polysaccharide.
Numerous outer and inner membrane, cytoplasmic, and periplasmic protein antigens have also been characterized. Some are reognized by the immune system during infection and are potentially useful in diagnostic tests (35). Hitherto, tests based on such antigens have suffered from low sensitivity as infected persons tend to develop a much less consistent response to individual protein antigens than to LPS. Thus, tests such as immunoblotting against whole-cell extracts may have some advantages over more quantitative tests that employ purified individual antigens (36).
Recently, ribosomal proteins have reemerged as immunologically important components. Interest in these first arose more than 20 years ago when crude ribosomal preparations were demonstrated to stimulate both antibody and cell-mediated responses and to confer protection against challenge with Brucella (37). However, the individual components responsible for such activity were not identified until recently. It has been established that the L7/L12 ribosomal proteins are important in stimulating cell-mediated responses. They elicit delayed hypersensivity responses as components of brucellins (38), and as fusion proteins, they have been shown to stimulate protective responses to Brucella (39). They appear to have potential as candidate vaccine components.
Mechanisms of Pathogenicity
Virulent Brucella organisms can infect both nonphagocytic and phagocytic cells. The mechanism of invasion of nonphagocytic cells is not clearly established. Cell components specifically promoting cell adhesion and invasion have not been characterized, and attempts to detect invasin genes homologous to those of enterobacteria have failed. Within nonphagocytic cells, brucellae tend to localize in the rough endoplasmic reticulum. In polymorphonuclear or mononuclear phagocytic cells, they use a number of mechanisms for avoiding or suppressing bactericidal responses. The S-LPS probably plays a substantial role in intracellular survival, as smooth organisms survive much more effectively than nonsmooth ones. Compared with enterobacterial LPS, S-LPS has many unusual properties: a relatively low toxicity for endotoxin-sensitive mice, rabbits, and chick embryos; low toxicity for macrophages; low pyrogenicity; and low hypoferremia-inducing activity. It is also a relatively poor inducer of interferon (and tumor necrosis factor) but, paradoxically, is an effective inducer of interleukin 12 (40,41).
S-LPS is the main antigen responsible for containing protection against infection in passive transfer experiments with monoclonal and polyclonal antibodies. The protection is usually short-term and incomplete, however. The elimination of virulent Brucella depends on activated macrophages and hence requires development of Th1 type cell-mediated responses to protein antigens (42).
An important determinant of virulence is the production of adenine and guanine monophosphate, which inhibit phagolysosome fusion; degranulation and activation of the myelo-peroxidase-halide system; and production of tumor necrosis factor (41,43). The production of these inhibitors is prevented in pur E mutants, which are substantially attenuated in consequence. Cu-Zn superoxide dismutase is believed to play a significant role in the early phase of intracellular infection (44). However, conflicting results have been reported, and this role needs to be confirmed.
Survival within macrophages is associated with the synthesis of proteins of molecular weight 17, 24, 28, 60, and 62 kDa. The 62 kDa protein corresponds to the Gro EL homologue Hsp 62, and the 60 kDa protein is an acidinduced variant of this. The 24 kDa protein is also acid-induced, and its production correlates with bacterial survival under acidic conditions (<pH4). The 17 and 28 kDa proteins are apparently specifically induced by macrophages and correlated with intracellular survival (45).
Another stress-induced protein, HtrA, is involved in the induction of an early granulomatous response to B. abortus in mice and is associated with a reduction in the levels of infection during the early phase. Howevr, it does not prevent a subsequent increase in bacterial numbers, and htrA-deficient mutants ultimately produce levels of splenic infection similar to those given by wild-type B. abortus (46). Similarly, recA-deleted mutants produce a lower initial spleen count than recA-positive strains but still establish persistent infection (47). The role of iron-sequestering proteins or other siderophores in the pathogenesis of brucellosis is still unknown. In general, the low availability of iron in vivo restricts microbial growth. However, high iron concentrations promote the killing of Brucella, probably by favoring production of hydroxylamine and hydroxyl radical.
The mechanisms of pathogenesis of Brucella infection in its natural host species and in humans are still not completely understood, and further studies are needed.
The clinical picture in human brucellosis can be misleading, and cases in which gastrointestinal, respiratory, dermal, or neurologic manifestations predominate are not uncommon (48-52). Because unusual cases with atypical lesions continue to be reported, diagnosis needs to be supported by laboratory tests (52). Blood culture is still the standard method and is often effective during the acute phase; the lysis concentration method gives the best results (53). Automated incubation-detection methods are effective, but allowance should be made for the relatively slow growth of the organism (54). Presumptive identification is made on the basis of morphologic, cultural, and serologic properties. Confirmation requires phage-typing, oxidative metabolism, or genotyping procedures. Reliance should not be placed on gallery type rapid identification systems as these have misidentified Brucella as Moraxella phenylpyruvica, with serious consequences for laboratory staff (55).
PCR with random or selected primers gives promising results, but standardization and further evaluation are needed, especially for chronic disease (56). Similarly, antigen detection methods are potentially useful but have not been validated. Combinations of these with PCR, such as immuno-PCR, have considerable potential but require evaluation. Enzyme immunoassay is now widely used for serologic diagnosis of the disease in humans and other species. IgA and IgG antibodies seem the most useful indicators of active infection (57,58). Western blotting against selected cytoplasmic proteins may be useful in support of screening tests to differentiate active from past or subclinical infection (35).
Despite extensive studies over the past 15 years, the optimum antibiotic therapy for brucellosis is still disputed. The treatment recommended by the World Health Organization for acute brucellosis in adults is rifampicin 600 to 900 mg and doxycycline 200 mg daily for a minimum of 6 weeks (59). Some still claim that the long-established combination of intramuscular streptomycin with an oral tetracycline gives fewer relapses (60). There is some evidence of physiologic antagonism between rifampicin and tetracyclines, but recent studies suggest that the two regimens have very similar results given adequate time. Quinolones in combination with rifampicin seem as effective as either of these regimens (61). Controlled clinical trials with other antibiotics, including new macrolides and ß-lactams, have either give inferior results or involved too few patients for proper evaluation.
Infections with complications, such as meningoencephalitis or endocarditis, require combination therapy with rifampicin, a tetracycline, and an aminoglycoside (62). Rifampicin has been recommended as the treatment of choice for uncomplicated disease in children, with cotrimoxazole as an alternative. Both are associated with a high relapse rate if used singly, and best results are achieved by using them in combination (63). Co-trimoxazole is an alternative but also has a high relapse rate. A combination of the two agents gives the best results.
Prevention of brucellosis in humans still depends on the eradication or control of the disease in animal hosts, the exercise of hygienic precautions to limit exposure to infection through occupational activities, and the effective heating of dairy products and other potentially contaminated foods. Vaccination now has only a small role in the prevention of human disease, although in the past, various preparations have been used, including the live attenuated B. abortus strains 19-BA and 104M (used mainly in the former Soviet Union and China), the phenol-insoluble peptidoglycan vaccine (formerly available in France), and the polysaccharide-protein vaccine (used in Russia). All had limited efficacy (64) and in the cases of live vaccines, were associated with potentially serious reactogenicity. Subunit vaccines against brucellosis are still of interest. The live vaccines have provoked unacceptable reactions in individuals sensitized by previous exposure to Brucella or if inadvertently administered by subcutaneous rather than percutaneous injection. These will probably require a combination of detoxified lipopolysaccharide-protein conjugate and protein antigens such as the L7/L12 ribosomal proteins presented in an adjuvant or delivery system favoring a Th1 type immune response. pur E mutants of B. melitensis appear safe in animals (65) and may have potential application as human vaccines if their safety and efficacy is confirmed in clinical trials. New vaccines have been evaluated for use in animals, including the B. suis strain 2 live vaccine given either orally or parenterally (66,67). This vaccine has proved inferior to the Rev.1. strain for the prevention of B. melitensis infection in sheep and goats and ineffective against B. ovis infection in sheep. B. abortus strain 19 still appears to be as effective as any for the prevention of B. abortus infection in cattle. However, the RB51 strain of B. abortus, an R mutant used as a live vaccine, has been licensed in the United States. This does not interfere with diagnostic serologic tests, but in laboratory trials, its efficacy appeared comparable with that of strain 19 (68). Similar rfb mutants of B. melitensis and B. suis are under development for the prevention of ovine/caprine and porcine brucellosis.
Substantial progress has been achieved in understanding the molecular basis of the genetics of Brucella and the pathogenesis of the infection. However, further progress is needed, especially in relation to diagnostic procedures and therapy. An effective and safe vaccine against human brucellosis is also some way in the future.
- Amato Gauci AJ. The return of brucellosis. Maltese Medical Journal. 1995;7:7–8.
- Garcia Carrillo C. Animal and human brucellosis in the Americas. Paris: OIE, 1990: 287.
- Lopez Merino A. Brucellosis in Latin America. Young EJ, Corbel MH, editors. Brucellosis; clinical and laboratory aspects. Boca Raton: CRC Press Inc., 1989:151-61.
- Mantur BG, Mangalgi SS, Mulimani B. Brucella melitensis-a sexually transmissible agent. Lancet. 1996;347:1763.
- Tessaro SV, Forbes LB. Brucella suis biotype 4; a case of granulomatous nephritis in a barren ground caribou (Rangifer tarandus groenlandicus L) with a review of the distribution of rangiferine brucellosis in Canada. J Wildl Dis. 1986;22:479–88.
- Carmichael LE. Brucella canis. In: Nielsen K, Duncan JR, editors. Animal brucellosis. Boca Raton: CRC Press Inc.: 1990,335-50.
- Ross HM, Foster G, Reid RJ, Jabans KL, MacMillan AP. Brucella species infection in sea mammals. Vet Rec. 1994;132:359.
- Ewalt DR, Payeur JB, Martin BM, Cummins DR, Miller WG. Characteristics of a Brucella species from a bottlenose dolphin (Tursiops truncatus). J Vet Diagn Invest. 1994;6:448–52.
- De Ley J, Mannheim W, Segers P, Lievens A. Ribosomal ribonucleic acid cistron similarities and taxonomic neighbourhood of Brucella and CDC Group Vd. Int J Syst Bacteriol. 1987;37:35–42.
- Verger JM, Grimont F, Grimont PAD, Grayon M. Brucella A monospecific genus as shown by deoxyribonucleic acid hybridization. Int J Syst Bacteriol. 1985;35:292–5.
- Alton GG, Jones LM, Pietz DE. Laboratory techniques in brucellosis. Geneva: World Health Organization 1975.
- Corbel MJ, Morgan WJB. Genus Brucella Meyer and Shaw 1920, 173 AL. In: Holt JG, editor. Bergey's manual of systematic bacteriology vol. 1. Baltimore (MD): Williams and Wilkins, 1984:377-88.
- Corbel MJ. Identification of dye-sensitive strains of Brucella melitensis. J Clin Microbiol. 1991;29:1066–8.
- Corbel MJ, Thomas EL. Use of phage for the identification of Brucella canis and Brucella ovis cultures. Res Vet Sci. 1985;35:35–40.
- Banai M, Mayer I, Cohen A. Isolation, identification and characterization in Israel of Brucella melitensis biovar 1 atypical strains susceptible to dyes and penicillin, indication of the evolution of a new variant. J Clin Microbiol. 1990;28:1057–9.
- Allardet-Servent A, Bourg G, Ramuz M, Bellis M, Roizes G. DNA polymorphism in strains of the genus Brucella. J Bacteriol. 1988;170:4603–7.
- O'Hara MJ, Collins DM, Lisle GW. Restriction endonuclease analysis of Brucella ovis and other Brucella species. Vet Microbiol. 1985;10:425–9.
- Ficht TA, Bearden SW, Sowa BA, Adams LG. DNA sequence and expression of the 36-kilodalton outer membrane protein gene of Brucella abortus. Infect Immun. 1989;57:3281–91.
- Cellier MFM, Teyssier J, Nicolas M, Liautard JB, Marti J. SriWidada J. Cloning and characterization of the Brucella ovis heat shock protein DnaK functionally expressed in Escherichia coli. J Bacteriol. 1992;174:8036–42.
- Sangari FJ, García-Lobo JM, Aguero J. The Brucella abortus vaccine strain B19 carries a deletion in the erythritol catabolic genes. FEMS Microbiol Lett. 1994;121:337–42.
- Douglas JT, Rosenberg EY, Nikaido H, Verstreate DR, Winter AJ. Porins of Brucella species. Infect Immun. 1984;44:16–21.
- Ficht TA, Husseinen HS, Derr J, Bearden SW. Species-specific sequences at the omp2 locus of Brucella type strains. Int J Syst Bacteriol. 1996;46:329–31.
- Cloeckaert A, Verger J-M, Grayon M, Grepinet O. Restriction site polymorphism of the genes encoding the major 25kDa and 36kDa outer membrane proteins of Brucella. Microbiology. 1995;141:2111–21.
- Cloeckaert A, Salih-Alj Debarrh H, Zygmunt MS, Dubray G. Polymorphism at the dnak locus of Brucella species and identification of a Brucella melitensis species-specific marker. J Med Microbiol. 1996;45:200–13.
- Michaux S, Paillisson J, Carles-Nurit MJ, Bourg G, Allardet Servent A, Ramuz M. Presence of two independent chromosomes in the Brucella melitensis 16M genome. J Bacteriol. 1993;175:701–5.
- Jumas-Bitlak E, Maugard C, Michaux-Charachon S, Allardet-Servent A, Perrin A, O'Callaghan D. Study of the organization of the genomes of Escherichia coli, Brucella melitensis and Agrobacterium tumefaciens by insertion of a unique restriction site. Microbiology. 1995;141:2425–32.
- Rigby CE, Fraser ADE. Plasmid transfer and plasmid-mediated genetic exchange in Brucella abortus. Can J Vet Res. 1989;53:326–30.
- Cieslak TJ, Robb ML, Drabick CJ, Fisher GW. Catheter-associated sepsis caused by Ochrobactrum anthropi: report of a case and review of related non-fermentative bacteria. Clin Infect Dis. 1992;14:902–7.
- Da Costa M, Guillou J-P, Garin-Bastuji B. ThiJbaud M, Dubray G. Specificity of six gene sequences for the detection of the genus Brucella by DNA amplification. J Appl Bacteriol. 1996;81:267–75.
- Minnick MF, Stiegler GL. Nucleotide sequence and comparison of the 5S ribosomal genes of Rochalimaea henselae, R. quintana and Brucella abortus. Nucleic Acids Res. 1993;21:2518.
- Relman DA, Lepp PW, Sadler KN, Schmidt TM. Phylogenetic relationships among the agent of bacillary angiomatosis, Bartonella bacilliformis, and other alpha-proteobacteria. Mol Microbiol. 1992;6:1801–7.
- Perry MB, Bundle DR. Lipopolysaccharide antigens and carbohydrates of Brucella. In: Adams LG, editor. Advances in Brucellosis Research Austin (TX): Texas A & M University, 1990;76-88.
- Bundle DR, Cherwonogrodzky JW, Gidney MAJ, Meikle PJ, Perry MB, Peters T. Definition of Brucella A and M epitopes by monoclonal typing reagents and synthetic oligosaccharides. Infect Immun. 1989;57:2829–36.
- Wilson GS, Miles AA. The serological differentiation of smooth strains of the Brucella group. Br J Exp Pathol. 1932;13:1–13.
- Goldbaum FA, Leoni J, Walach JC, Fossati CA. Characterisation of an 18-kilodalton Brucella cytoplasmic protein which appears to be a serological marker of active infection of both human and bovine brucellosis. J Clin Microbiol. 1993;31:2141–5.
- Goldbaum FA, Morelli L, Wallach J, Rubbi CP, Fossati CA. Human brucellosis: immunoblotting analysis of three Brucella abortus antigenic fractions allows the detection of components of diagnostic importance. Medicina (B Aires). 1991;51:227–32.
- Corbel MJ. The immunogenic activity of ribosomal fractions derived from Brucella abortus. Journal of Hygiene, Cambridge. 1976;76:65–74.
- Bachrach G, Banai M, Bardenstein S, Hoida G, Genizi A, Bercovier H. Brucella ribosomal protein L7 / L12 is a major component in the antigenicity of Brucellin INRA for delayed hypersensitivity in Brucella-sensitized guinea- pigs. Infect Immun. 1994;62:5361–6.
- Oliveira S, Splitter GA. Immunization of mice with recombinant L7 / L12 ribosomal protein confers protection against Brucella abortus infection. Vaccine. 1996;14:959–62.
- Zhan Y, Cheers C. Differential activation of Brucella-reactive CD4+ cells by Brucella infection or immuni-zation with antigenic extracts. Infect Immun. 1995;63:969–95.
- Caron E, Peyrard T, Kohler S, Cabane S, Liautard J-P, Dornand J. Live Brucella spp. fail to induce tumour necrosis factor alpha excretion upon infection of U937-derived phagocytes. Infect Immun. 1994;62:5267–74.
- Dubray G. Protective antigens in brucellosis. Ann Inst Pasteur Microbiol. 1987;138:84–7.
- Canning PC, Roth JA, Deyoe BL. Release of 5'-guanosine monophosphate and adenine by Brucella abortus and the intracellular survival of the bacteria. J Infect Dis. 1986;154:464–70.
- Bricker BJ, Tabatabai LB, Judge BA, Deyoe BL, Mayfield JE. Cloning, expression and occurrence of the Brucella Cu-Zn dismutase. Infect Immun. 1990;58:2933–9.
- Lin J, Ficht TA. Protein synthesis in Brucella abortus induced during macrophage infection. Infect Immun. 1995;63:1409–14.
- Tatum FM, Cheville NF, Morfitt D. Cloning, charac-terisation and construction of htr A and htr A - like mutants of Brucella abortus and their survival in BALB/C mice. Microb Pathog. 1994;17:23–36.
- Tatum FM, Morfitt DC, Halling SM. Construction of a Brucella abortus Rec A mutant and its survival in mice. Microb Pathog. 1993;14:177–85.
- Santini C, Baiocchi P, Berardelli A, Venditti M, Serra P. A case of brain abscess due to Brucella melitensis. Clin Infect Dis. 1994;19:977–8.
- Potasman I, Even L, Banai M, Cohen E, Angel D, Jaffe M. Brucellosis: an unusual diagnosis for a seronegative patient with abscesses, osteomyelitis and ulcerative colitis. Rev Clin Dis. 1991;13:1039–42.
- Shakir RA, Al-Din ASN, Araj GF, Lulu AR, Mousa AR, Saadah MA. Clinical diagnosis of neurobrucellosis. A report on 19 cases. Brain. 1987;110:213–23.
- Young EJ. An overview of human brucellosis. Clin Infect Dis. 1995;21:283–90.
- Madkour MM. Brucellosis. Butterworths, London 1989.
- Kolman S, Maayan MC, Gotesman G, Roszenstain LA, Wolach B, Lang R. Comparison of the Bactec and lysis concentration method for the recovery of Brucella species from clinical specimens. Eur J Clin Microbiol Infect Dis. 1991;10:647–8.
- Solomon HM, Jackson D. Rapid diagnosis of Brucella melitensis in blood; some operational characteristics of the BACT / ALERT. J Clin Microbiol. 1992;28:2139–41.
- Luzzi GA, Brindle R, Socket PN, Solera J, Klenerman P, Warrell DA. Brucellosis: imported and laboratory-acquired cases, and an overview of treatment trials. Trans R Soc Trop Med Hyg. 1993;87:138–41.
- Matar FM, Khreissir IA, Abdonoor AM. Rapid laboratory confirmation of human brucellosis by PCR analysis of a target sequence on the 31-kilodalton Brucella antigen DNA. J Clin Microbiol. 1996;34:477–8.
- Araj GF, Lulu AR, Mustafa MY, Khateeb MI. Evaluation of ELISA in the diagnosis of acute and chronic brucellosis in human beings. Journal of Hygiene, Cambridge. 1986;97:457–69.
- Ariza J, Pellicer T, Pallarés R, Foz A, Gudiol F. Specific antibody profile in human brucellosis. Clin Infect Dis. 1992;14:131–40.
- Joint FAO, Expert WHO. Committee on Brucellosis. Sixth Report. World Health Organ Tech Rep Ser No. 740. Geneva: World Health Organization, 1986.
- Ariza J, Gudiol F, Pallarés R, Rufi G, Fernàndez-Viladrich P. Comparative trial of rifampicin-doxycycline versus tetracycline-streptomycin in the therapy of human brucellosis. Antimicrob Agents Chemother. 1985;28:548–51.
- Akova M, Uzun O, Akalin HE, Hayran M, Unal S, Gur D. Quinolones in the treatment of human brucellosis; comparative trial of ofloxacin-rifampin versus doxycycline- rifampin. J Antimicrob Chemother. 1993;37:1831–4.
- Shakir RA. Postgrad Med J. 1986;62:1077–9.Neurobrucellosis
- Khuri-Bulos NA, Daoud AH, Azab SM. Treatment of childhood brucellosis: results of a prospective trail on 113 childred. Pediatr Infect Dis. 1993;12:377–81.
- Corbel MJ. Vaccines against bacterial zoonoses. J Med Microbiol. 1997;46:267–9.
- Crawford RM, Van De Verg L, Yuan L, Hadfield TL, Warren RL, Drazek ES, Deletion of purE attenuates Brucella melitensis infection in mice. Infect Immun. 1996;64:2188–92.
- Xie X. Orally administered brucellosis vaccine Brucella suis strain 2 vaccine. Vaccine. 1986;4:212–6.
- Mustafa AA, Abusowa M. Field-oriented trial of the Chinese Brucella suis strain 2 vaccine in sheep and goats in Libya. Annales de Recherche Veterinaire. 1993;24:422–9.
- Schurig GG, Roop RM, Bagchi T, Boyle S, Buhrman D, Sriranganathan N. Biological properties of RB51. A stable strain of Brucella abortus. Vet Microbiol. 1991;28:171–88.
Suggested citation: Corbel MJ. Brucellosis: an Overview. Emerg Infect Dis [serial on the Internet]. 1997, Jun [date cited]. Available from http://wwwnc.cdc.gov/eid/article/3/2/97-0219.htm
West Nile Virus RNA
in Tissues from Donor
Transmission to Organ