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Volume 4, Number 2—June 1998


Mycobacterium tuberculosis infection as a Zoonotic Disease: Transmission between Humans and Elephants

Kathleen Michalak*, Connie Austin†, Sandy Diesel*, J. Maichle Bacon*, Phil Zimmerman‡, and Joel N. Maslow§
Author affiliations: *McHenry County Department of Health, Woodstock, Illinois, USA; †Illinois Department of Public Health, Springfield, Illinois, USA; ‡University of Illinois, College of Medicine at Rockford, Rockford, Illinois, USA; §Boston University School of Medicine and the VA Medical Center, Boston, Massachusetts, USA

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Figure 1

Number of erythromycin-resistant blood and cerebro- spinal fluid isolates of pneumococci. DNA was extracted from pneumococcal isolates by using a lysis solution consisting of 0.1% sodium deoxycholate as described in (11), except that we used plate rather than broth cultures. 
 Seventy-eight MLS strains were probed for the ermAM gene by using dot blots. The probe (supplied by P. Courvalin, Pasteur Institute, Paris, France) (Escherichia coli JM83/pUC19 560bp Ssp1 intragenic fragment of ermB) was l

Figure 1. IS6110 restriction fragment length polymorphism results. Lane 1, elephant isolate (died August 6, 1996); Lane 2, elephant isolate (died 1994); Lane 3, living elephant trunk culture (October 1996); Lane 4, elephant lung tissue isolate (died August 3, 1996); Lane 5, elephant lymph node tissue isolate (died August 3, 1996); Lane 6, human sputum isolate (September 1996). Provided by State of Michigan Community Public Health Agency.

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