Figure. A. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the 16S rRNA gene of B. vinsonii subsp. berkhoffii isolates using Dde I demonstrating differentiation between subspecies of B. vinsonii. B. PCR-RFLP analysis of the 16S-23S intergenic spacer region of B. vinsonii subsp. berkhoffii isolates using Hae III. Strain differences were detected between B. vinsonii subsp. berkhoffii (T), which was the type strain cultured from a dog with endocarditis, and isolates A (described in this report), B, and C from subclinically infected dogs.
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