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Volume 5, Number 2—April 1999

Research

Rapid Molecular Genetic Subtyping of Serotype M1 Group A Streptococcus Strains

Nancy Hoe*, Kazumitsu Nakashima*, Diana Grigsby*, Xi Pan*, Shu Jun Dou*, Steven Naidich†, Marianne Garcia‡, Emily Kahn‡, David Bergmire-Sweat‡, and James M. Musser*Comments to Author 
Author affiliations: *Baylor College of Medicine, Houston, Texas, USA; †Naidich Space Laboratory, Inc., New York, New York, USA; ‡Texas Department of Health, Austin, Texas, USA

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Figure 3

Representative IS1548 RFLP fingerprint patterns of M1 isolates. Panel A is a lane map showing results from analysis of three serotype M3 control isolates and four M1 isolates with different sic alleles. Lane 1, MGAS5892; lane 2, MGAS6004; lane 3, MGAS6005; lane 4, MGAS5997; lane 5, MGAS5999; lane 6, MGAS6003; lane 7, MGAS6006. kb, 1-kb DNA ladder. Panel B is a lane map showing results from analysis of eleven M1 isolates with eight different sic alleles. Lane 1, MGAS6201; lane 2, MGAS6249; lane 3

Figure 3. Representative IS1548 RFLP fingerprint patterns of M1 isolates. Panel A is a lane map showing results from analysis of three serotype M3 control isolates and four M1 isolates with different sic alleles. Lane 1, MGAS5892; lane 2, MGAS6004; lane 3, MGAS6005; lane 4, MGAS5997; lane 5, MGAS5999; lane 6, MGAS6003; lane 7, MGAS6006. kb, 1-kb DNA ladder. Panel B is a lane map showing results from analysis of eleven M1 isolates with eight different sic alleles. Lane 1, MGAS6201; lane 2, MGAS6249; lane 3, MGAS6251; lane 4, MGAS6254; lane 5, MGAS6262; lane 6, MGAS6264; lane 7, MGAS6272; lane 8, MGAS6281; lane 9, MGAS6293; lane 10, MGAS6294; lane 11, MGAS6299. kb, 1-kb DNA ladder. Panel C is a lane map showing results from analysis of four speA-positive and seven speA-negative M1 isolates. Lane 1, MGAS2350, lane 2, MGAS2221, lane 3, MGAS2139, lane 4, MGAS1272, lane 5, MGAS6708, lane 6, MGAS1653, lane 7, MGAS1642, lane 8, MGAS1632, lane 9, MGAS570, lane 10, MGAS326, lane 11, MGAS279. kb, 1-kb DNA ladder.

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