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Volume 5, Number 2—April 1999

Research

Rapid Molecular Genetic Subtyping of Serotype M1 Group A Streptococcus Strains

Nancy Hoe*, Kazumitsu Nakashima*, Diana Grigsby*, Xi Pan*, Shu Jun Dou*, Steven Naidich†, Marianne Garcia‡, Emily Kahn‡, David Bergmire-Sweat‡, and James M. Musser*Comments to Author 
Author affiliations: *Baylor College of Medicine, Houston, Texas, USA; †Naidich Space Laboratory, Inc., New York, New York, USA; ‡Texas Department of Health, Austin, Texas, USA

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Table

Characteristics of serotype M1 Group A Streptococcus isolates analyzed

MGAS no.a TDH no.b sic allele emm1 allele DRc PCRd (bp) DR sequence type speA PCRe IS1548 type
6151 BE8-776 1.01 1.0 372 4.0 pos 1.0
6168 BE-98-743 1.01 1.0 306 3.0 pos 1.0
6184 BE8-873 1.01 1.0 306 NSf pos 1.0
6199 BE8-917 1.01 1.19 306 NS pos 1.0
6262 BE8-1085 1.01 1.19 306 NS pos 1.0
6264 BE8-1087 1.01 1.19 306 3.0 pos 1.0
6181 BE-98-764 1.02 1.0 240 NS pos 1.0
6293 BE8-1339 1.02 1.0 306 NS pos 1.0
6294 BE8-1340 1.02 1.0 306 NS pos 1.4
6140 BE8-629 1.13 1.0 240 NS pos 1.0
6200 BE8-918 1.13 1.0 240 NS pos 1.0
6201 BE8-919 1.13 1.0 240 NS pos 1.0
6281 BE8-1149 1.13 1.24 306 NS pos 1.3
6137 BE8-563 1.32 1.0 306 3.0 pos 1.0
6148 BE8-773 1.32 1.0 306 NS pos 1.0
6249 BE8-929 1.32 1.0 306 NS pos 1.0
6172 BE-98-751 1.34 1.0 306 NS pos 1.0
5997 BE8-191 1.36 1.0 240 NS pos 1.0
6135 BE8-548 1.36 1.0 240 2.2 pos 1.0
6254 BE8-1021 1.36 1.24 306 NS pos 1.0
6189 BE8-88 1.66 1.13 306 NS pos 1.0
5999 BE8-208 1.99 1.0 306 3.0 pos 1.0
6003 BE8-322 1.100 1.0 240 NS pos 1.0
6251 BE8-1000 1.100 1.0 240 2.1 pos 1.0
6006 BE8-369 1.101 1.0 306 3.0 pos 1.0
6138 BE8-566 1.118 1.0 240 2.2 pos 1.0
6150 BE8-775 1.119 1.0 306 3.0 pos 1.0
6154 BE8-792 1.120 1.18 240 2.1 pos 1.0
6272 BE8-1111 1.179 1.0 306 NS pos 1.0
6299 BE8-1380 1.180 1.0 240 2.0 pos 1.0
2221 NA 1.01 1.0 306 NS pos 1.0
5305 NA 1.01 1.0 306 3.0 pos 1.0
5809 NA 1.01 1.0 305 3.01 pos 1.0
2139 NA 1.02 1.0 306 3.0 pos 1.0
2350 NA 1.09 1.0 306 3.0 pos 1.0
1272 NA 1.35 1.0 306 NS pos 1.5
5297 NA 1.121 1.0 240 2.0 pos 1.0
279 NA 1.08 1.3 570 7.0 neg 1.6
1632 NA 1.08 1.3 570 7.0 neg 1.6
1653 NA 1.19 1.3 570 7.0 neg 1.6
326 NA 1.20 1.3 570 7.0 neg 1.6
570 NA 1.21 1.3 570 7.0 neg 1.8
1642 NA 1.24 1.3 504 6.1 neg 1.6
6708g NA 1.225 1.6 504 6.0 neg 1.7

aMGAS, Musser group A Streptococcus strain number. All isolates had no known direct epidemiologic connection except MGAS 6199, 6264, and 6272 (associated household cases); MGAS 6140, 6200, and 6201 (blood and cerebrospinal fluid cultures of same patient); and MGAS 6293 and 6294 (mother-neonate paired isolates).
bTDH, Texas Department of Health strain number; NA, not applicable (control isolate).
cDR, direct repeat.
dPCR, polymerase chain reaction.
epos, PCR-positive for speA; neg, PCR-negative for speA. The speA gene in MGAS 1272, 6135, 6137, 6138, 6150, 6151, 6154, 6168, 6251, 6264, 6272, and 6299 was sequenced and identified as allele speA2.
fNS, not sequenced.
gMGAS 6708 is also known as SF370. The genome of this organism is being sequenced at the University of Oklahoma.

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