Emerging Infectious Disease ISSN: 1080-6059

Neospora caninum Infection and Repeated Abortions in Humans

Eskild Petersen*

, Morten Lebech*, Lene Jensen†, Peter Lind†, Martin Rask†, Peter Bagger‡, Camilla Björkman§, and Arvid Uggla§

Author affiliations: *Statens Serum Institut, Copenhagen, Denmark; †State Veterinary Laboratory, Copenhagen, Denmark; ‡Rigshospitalet, Copenhagen, Denmark; §Swedish University of Agricultural Sciences, Uppsala, Sweden

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Western blot of Toxoplasma gondii or Neospora caninum antigen. Analysis was performed essentially as described by Sharma et al. (12) by using tachyzoites from in vitro culture of the N. caninum NC-1 isolate (13) and the T. gondii RH strain (10). Lanes 1-4 were probed with control sera and lanes 5-12 with human sera with high absorbencies in the N. caninum enzyme-linked immunosorbent assay.  Lane 1: T. gondii-positive human serum and T. gondii antigen; Lane 2: N. caninum-positive pig serum and T.

Figure. Western blot of Toxoplasma gondii or Neospora caninum antigen. Analysis was performed essentially as described by Sharma et al. (12) by using tachyzoites from in vitro culture of the N. caninum NC-1 isolate (13) and the T. gondii RH strain (10). Lanes 1-4 were probed with control sera and lanes 5-12 with human sera with high absorbencies in the N. caninum enzyme-linked immunosorbent assay.  Lane 1: T. gondii-positive human serum and T. gondii antigen; Lane 2: N. caninum-positive pig serum and T. gondii antigen; Lane 3: T. gondii-positive human serum and N. caninum antigen; Lane 4: N. caninum-positive pig serum and N. caninum antigen; Lane 5 and 6: Serum 262: T. gondii and N. caninum antigens, respectively; Lane 7 and 8: Serum 264: T. gondii and N. caninum antigens, respectively; Lane 9 and 10: Serum 276: T. gondii and N. caninum antigens, respectively. Lane 11 and 12: Serum 279: T. gondii and N. caninum antigens, respectively.

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