Volume 5, Number 2—April 1999
Neospora caninum Infection and Repeated Abortions in Humans
|Sample no.||ELISA mean OD||Dye test|
aThe ELISA was modified from Björkman et al. (8,9). A T. gondii-negative human serum specimen was used as a presumed N. caninum-negative control, and serum specimens from experimentally N. caninum-infected pigs were used as positive controls. A low-positive serum was collected from a pig infected 11 days before sampling and a high-positive control serum was pooled from pigs infected for at least 3 weeks (10). No reaction in human sera was definitely positive.
bThe dye test was used to demonstrate antibodies to T. gondii as described (11) but using in vitro cultured T. gondii.