Volume 5, Number 5—October 1999
Synopsis
Morphologic and Molecular Characterization of New Cyclospora Species from Ethiopian Monkeys: C. cercopitheci sp.n., C. colobi sp.n., and C. papionis sp.n.
Mark L. Eberhard*
, Alexandre J. da Silva*, Bruce G. Lilley†, and Norman J. Pieniazek*
, Alexandre J. da Silva*, Bruce G. Lilley†, and Norman J. Pieniazek*Table
New Cyclospora species from Ethiopian monkeys
| Characteristics | Cyclospora cercopitheci | C. colobi | C. papionis |
|---|---|---|---|
| Host | Cercopithecus aethiops Linnaeus, 1758, African green or vervet monkey | Colobus guereza Ruppell, 1835, colobus monkey | Papio anubis Lesson, 1827, olive baboon |
| Oocysts | Spherical; 8 - 10 µm (mean 9.2) in diameter. Outer wall smooth. Wall autofluoresces in UV wavelength. | Small, spherical, 8 - 9 µm (mean 8.3) in diameter. Outer wall smooth. Wall autofluoresces in UV wavelength. | Spherical, 8 - 10 µm (mean 8.8) in diameter. Outer wall smooth. Wall autofluoresces in UV wavelength. |
| Sporocysts | Two per mature oocyst Lemon-shaped, 6-7 by 4-5 µm, with L/W ratio 1.5 | Two per mature oocyst Lemon-shaped, 7-8 by 4-5 µm, with L/W ratio 1.66 | Two per mature oocyst Lemon-shaped, 7-8 by 4-5 µm, with L/W ratio ratio 1.66 |
| Stieda bodies | A prominent stieda body present; sub- and parastieda bodies absent | A prominent stieda body present; sub- and parastieda bodies absent | A prominent stieda body present; sub- and parastieda bodies absent |
| Sporocyst residuum | Prominent; made up of clumped globules | Prominent, irregularly shaped; 2-4 µm in diameter | Prominent, irregularly shaped; 2-3 by 3-4 µm in diameter |
| Micropyle | Absent | Absent | Absent |
| Sporozoites | Two per sporocyst 10-13 by 1.5 µm; tapered at both ends | Two per sporocyst 10-13 by 2 µm; tapered at both ends | Two per sporocyst 10-13 by 1.5 µm; tapered at both ends |
| Remarks | Marginally larger than other two species Heterozygotic position, T or A at position #280; therefore, SSU-rRNA sequences submitted separately. Assigned accession nos. AF111184 and AF111185 | Marginally smaller than the two other species Sequence of SSU-rRNA assigned accession no. AF111186. Poorest sporulation rate of three species | The most commonly encountered of the three species. Sequence of SSU-rRNA assigned accession no. AF111187 |
