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Volume 6, Number 2—April 2000

Synopsis

The bdr Gene Families of the Lyme Disease and Relapsing Fever Spirochetes: Potential Influence on Biology, Pathogenesis, and Evolution

David M. Roberts*, Jason A Carlyon†, Michael Theisen, and Richard T. Marconi*Comments to Author 
Author affiliations: *Medical College of Virginia at Virginia Commonwealth University, School of Medicine, Richmond, Virginia, USA; †Yale School of Medicine, Yale University, New Haven, Connecticut, USA; ‡Statens Serum Institute, Copenhagen, Denmark

Main Article

Figure 1

Restriction fragment length polymorphism pattern analysis of the rep or bdr genes of the Lyme disease spirochetes. Total DNA, isolated from Borrelia cultures, was digested with Xba1, fractionated by electrophoresis, and transferred onto membranes for hybridization. Hybridization was performed by the bdrAB-R1 oligonucleotide (46). The species and isolates analyzed are indicated above each lane. MW markers in kb are indicated.

Figure 1. Restriction fragment length polymorphism pattern analysis of the rep or bdr genes of the Lyme disease spirochetes. Total DNA, isolated from Borrelia cultures, was digested with Xba1, fractionated by electrophoresis, and transferred onto membranes for hybridization. Hybridization was performed by the bdrAB-R1 oligonucleotide (46). The species and isolates analyzed are indicated above each lane. MW markers in kb are indicated.

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