Figure 1. Restriction fragment length polymorphism pattern analysis of the rep or bdr genes of the Lyme disease spirochetes. Total DNA, isolated from Borrelia cultures, was digested with Xba1, fractionated by electrophoresis, and transferred onto membranes for hybridization. Hybridization was performed by the bdrAB-R1 oligonucleotide (46). The species and isolates analyzed are indicated above each lane. MW markers in kb are indicated.