Emerging Infectious Disease ISSN: 1080-6059

Emerging Chagas Disease: Trophic Network and Cycle of Transmission of Trypanosoma cruzi from Palm Trees in the Amazon

Antonio R.L. Teixeira*

, Pedro Sadi Monteiro*, José M. Rebelo†, Enrique R. Argañaraz*, Daniela Vieira*, Liana Lauria-Pires*, Rubens Nascimento*, Cássia A. Vexenat*, Antonio R. Silva†, Steven K. Ault‡, and Jackson M. Costa‡

Author affiliations: *University of Brasília,; †Federal University of Maranhão,; ‡Pan-American Health Organization/World Health Organization, Brasília, Brazil

Main Article

Figure 7

In situ hybridization of Rhodnius pictipes-derived flagellates with an archetypic Berenice Trypanosoma cruzi-specific probe. The byotinylated 198-bp sequence amplified with Tcz1/2 nuclear DNA probe (37) was revealed with fluorescein-conjugated streptavidin. This genotypic marker, representing 12.5% of total parasitic nuclear DNA, also confirms this sylvatic isolate as T. cruzi.

Figure 7. . In situ hybridization of Rhodnius pictipes-derived flagellates with an archetypic Berenice Trypanosoma cruzi-specific probe. The byotinylated 198-bp sequence amplified with Tcz1/2 nuclear DNA probe (37) was revealed with fluorescein-conjugated streptavidin. This genotypic marker, representing 12.5% of total parasitic nuclear DNA, also confirms this sylvatic isolate as T. cruzi.

Main Article

¹In this study, a major ecosystem is defined as a set of ecoregions of comparable dynamics, response characteristics to disturbance, species diversity, and conservation needs. An ecoregion is a geographically distinct set of natural communities with similar species, ecologic dynamics, environmental conditions, and ecologic interactions critical for long-term persistence (1).

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