Emerging Infectious Disease ISSN: 1080-6059

Transferable Plasmid-Mediated Resistance to Streptomycin in Clinical Isolate of Yersinia pestis

Annie Guiyoule*, Guy Gerbaud*, Carmen Buchrieser*, Marc Galimand*, Lila Rahalison†, Suzanne Chanteau†, Patrice Courvalin*, and Elisabeth Carniel*

Author affiliations: *Institut Pasteur, Paris, France; †Institut Pasteur, Antananarivo, Madagascar

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Figure 1

Analysis of plasmid pIP1203. A) Agarose-gel electrophoresis of EcoRV-digested plasmid DNA from representative Yersinia pestis strain 6/69 (1) and from streptomycin-resistant strain 16/95 (2). B) Pulsed-field gel electrophoresis of pIP1203 DNA extracted from Escherichia coli K802N transconjugant and digested by EcoRV (1), EcoRI+EcoRV (2), and EcoRI (3). The arrow indicates the extra large-size DNA fragment in strain 16/95. The size of the molecular weight markers in kilobases is indicated at the

Figure 1. Analysis of plasmid pIP1203. A) Agarose-gel electrophoresis of EcoRV-digested plasmid DNA from representative Yersinia pestis strain 6/69 (1) and from streptomycin-resistant strain 16/95 (2). B) Pulsed-field gel electrophoresis of pIP1203 DNA extracted from Escherichia coli K802N transconjugant and digested by EcoRV (1), EcoRI+EcoRV (2), and EcoRI (3). The arrow indicates the extra large-size DNA fragment in strain 16/95. The size of the molecular weight markers in kilobases is indicated at the left of the gels.

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