Volume 7, Number 1—February 2001
Research
Transferable Plasmid-Mediated Resistance to Streptomycin in Clinical Isolate of Yersinia pestis
Annie Guiyoule*, Guy Gerbaud*, Carmen Buchrieser*, Marc Galimand*, Lila Rahalison†, Suzanne Chanteau†, Patrice Courvalin*, and Elisabeth Carniel*
Table 1
Bacterial strain used in study of resistant Yersinia pestis isolate 16/95
| Characteristics | ||
|---|---|---|
| Strain | and plasmid contenta | Source or reference |
| Y. pestis | ||
| 16/95 | pFra, pPla, pYV, pIP1203 Tra+b Smc | Wild strain |
| 6/69 | pFra, pPla, pYV | Wild strain |
| 6/69cN | Nald, pFra, pPla | Spontaneous Nal mutant of pYV cured 6/69 |
| 6/69cNR | Nal, Rife, pFra, pPla | Spontaneous Rif mutant of 6/69cN |
| 6/69cN(pIP1203) | Nal, pFra, pPla, pIP1203 Tra+ Sm | Transconjugant 16/95 x 6/69cN |
| Y. pseudotuberculosis | ||
| IP32790 | pYV | Wild strain |
| IP32790cN | Nal | Spontaneous Nal mutant of pYV cured IP32790 |
| IP32790cN(pIP1203) | Nal, pIP1203 Tra+ Sm | Transconjugant 16/95 x IP32790cN |
| Y. enterocolitica | ||
| IP864 | pYV | Wild strain |
| IP864cN | Nal | Spontaneous Nal mutant of pYV cured IP864 |
| Escherichia coli | ||
| C600R | thr leuB6 thi-1 lacY supE rpoB | Spontaneous Rif mutant of C600, Bachmann (9) |
| JM109 | hsdR- supE gyrA | Yanisch-Perron et al. (10) |
| K802N | hsdR- hsdM+ gal- met- supE gyrA | Wood (11) |
| K802N(pIP1203) | pIP1203 Tra+ Sm | Transconjugant 16/95 x K802N |
aPlasmid content = pFRa, pPla, and pYV are the endogenous plasmids of Y pestis (12).
bTra+ = self-transferable.
cSm = streptomycin resistance.
dNal = nalidixic acid resistance.
eRif = rifampicin resistance.
