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Volume 7, Number 1—February 2001

Research

Nested Polymerase Chain Reaction for Amplification of the Cryptosporidium Oocyst Wall Protein Gene

Susana Pedraza-Díaz*, Corinne Amar*, Gordon L. Nichols†, and Jim McLauchlin*Comments to Author 
Author affiliations: *PHLS Central Public Health Laboratory, London, UK; †PHLS Communicable Disease Surveillance Centre, London, UK

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Table 2

Cryptosporidium oocyst wall protein (COWP) gene analysis of DNA extracted from 1,680 human fecal samples

PCR amplification procedure No. of samples (%) 1 2 1 & 2 3
COWP gene fragment amplified
Unnested 1,304 (77.6) 381 917 2 4
Nesteda 296 (17.6) 81 198 7 10
COWP gene fragment not amplifiedb
Oocysts detected by microscopy 35 (2.1)
Microscopy not reconfirmedc 2 (0.1)
Oocysts not reconfirmed by microscopy 43 (2.6)

aAll samples previously negative by unnested procedure.
bBy both unnested and nested procedures.
cInsufficient material available.

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