Volume 7, Number 1—February 2001
Research
Nested Polymerase Chain Reaction for Amplification of the Cryptosporidium Oocyst Wall Protein Gene
Susana Pedraza-Díaz*, Corinne Amar*, Gordon L. Nichols†, and Jim McLauchlin*
Table 2
Cryptosporidium oocyst wall protein (COWP) gene analysis of DNA extracted from 1,680 human fecal samples
| PCR amplification procedure | No. of samples (%) | 1 | 2 | 1 & 2 | 3 |
|---|---|---|---|---|---|
| COWP gene fragment amplified | |||||
| Unnested | 1,304 (77.6) | 381 | 917 | 2 | 4 |
| Nesteda | 296 (17.6) | 81 | 198 | 7 | 10 |
| COWP gene fragment not amplifiedb | |||||
| Oocysts detected by microscopy | 35 (2.1) | ||||
| Microscopy not reconfirmedc | 2 (0.1) | ||||
| Oocysts not reconfirmed by microscopy | 43 (2.6) | ||||
aAll samples previously negative by unnested procedure.
bBy both unnested and nested procedures.
cInsufficient material available.
