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Volume 7, Number 1—February 2001

Research

Nested Polymerase Chain Reaction for Amplification of the Cryptosporidium Oocyst Wall Protein Gene

Susana Pedraza-Díaz*, Corinne Amar*, Gordon L. Nichols†, and Jim McLauchlin*Comments to Author 
Author affiliations: *PHLS Central Public Health Laboratory, London, UK; †PHLS Communicable Disease Surveillance Centre, London, UK

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Table 3

Cryptosporidium oocyst wall protein (COWP) gene analysis of DNA from 2,057 humans and 71 livestock animals

Humans 1 2 1 & 2 3 Reference
2,057 cases 795 1,227 20 15 This study
Drinking water-associated outbreaks
1 140 2 3 0 20,21
2 158 14 1 1 22
3 4 0 0 0 20
4 15 0 0 0 23
5 0 6 0 0 24
6 0 25 0 0 24
7 4 331 0 2 24
Swimming pool-associated outbreaks
8 3 0 0 0 24
9 6 3 0 0 25
10 0 10 0 0 25
11 9 1 3 0 25
12 14 2 0 0 25
Sporadic human cases
442 833 13 12 8, this study
Livestock animals
Calves, lambs 0 71 0 0 This study

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