Volume 7, Number 1—February 2001
Research
A Flea-Associated Rickettsia Pathogenic for Humans
Didier Raoult*
, Bernard La Scola*, Maryse Enea*, Pierre-Edouard Fournier*, Véronique Roux*, Florence Fenollar*, Marcio A.M. Galvao†, and Xavier de Lamballerie*
, Bernard La Scola*, Maryse Enea*, Pierre-Edouard Fournier*, Véronique Roux*, Florence Fenollar*, Marcio A.M. Galvao†, and Xavier de Lamballerie*Table 1
Primers used for PCR amplification and sequencing of the ELB agent strain Marseille-URRWFXCal2
| Gene position relative | ||||
|---|---|---|---|---|
| Gene | Primer | Nucleotide sequence (5'-3') | to open reading frame | Reference |
| 17 kDa antigen | 17kDFa | GCTCTTGCAACTTCTATGTT | 31-50 | This manuscript |
| 17KdRa | CATTGTTCGTCAGGTTGGCG | 464-445 | This manuscript | |
| Citrate synthase | CSFEL1a | TGATTCAGAATTTGCCGAAT | 21-40 | 17 |
| CS890ra | GCTTTAGCTACATATTTAGG | 890-871 | 17 | |
| CS532ra | GCCGCAATGTCTTATAAATATTCT | 532-555 | This manuscript | |
| CS1273ra | CATAACCAGTGTAAAGCTG | 1273-1255 | This manuscript | |
| CS244rb | CTTTAATATCATATCCTCGAT | 244-224 | 17 | |
| Rp877Pb | GGGGACCTGCTCACGGCGG | 797-815 | 18 | |
| rOmpB | 120-M59a | CCGCAGGGTTGGTAACTGC | M59-M41 | This manuscript |
| 120-807a | CCTTTTAGATTACCGCCTAA | 807-788 | This manuscript | |
| 120-607a | AATATCGGTGACGGTCAAGG | 607-626 | This manuscript | |
| 120-1497a | CTATATCGCCGGTAATT | 1497-1480 | This manuscript | |
| 120F-1351a | TTTAGGAAACGCTGGTTCTC | 1351-1370 | This manuscript | |
| 120F-2934a | GCGTTAGTTGCGATAATACT | 2934-2915 | This manuscript | |
| 120-2788a | AAACAATAATCAAGGTACTGT | 2788-2808 | This manuscript | |
| 120-3599a | TACTTCCGGTTACAGCAAAGT | 3599-3579 | This manuscript | |
| 120F-3440a | GTTAATGCAACAACTACGGG | 3440-3459 | This manuscript | |
| 120F-4341a | GCATCGAAGAAGTAACGCTG | 4341-4322 | This manuscript | |
| 120-4232a | GGTTTCTCATTCTCTCTATATGG | 4232-4254 | This manuscript | |
| 120-4879a | TTAGAAGTTTACACGGACTTT | 4879-4857 | This manuscript | |
| 120F-1749b | CTATTAGTGGTAATATTGGTAC | 1749-1770 | This manuscript | |
| 120F-2495b | CATGGTCATTACCTGCATTACC | 2495-2481 | This manuscript | |
| 120-4489b | GTCTTCTGACGAAAACTACAA | 4489-4509 | This manuscript | |
| ROmpA | 190-70a | ATGGCGAATATTTCTCCAAAA | 70-90 | 18 |
| 190-701a | GTTCCGTTAATGGCAGCATCT | 701-681 | 19 |
aPrimers used for both PCR amplification and sequencing of the ELB agent strain Marseille-URRWFXCal2.
bPrimers used only for sequencing.
