Volume 7, Number 2—April 2001
4th Decennial International Conference on Nosocomial and Healthcare-Associated Infections
State of the Art
Molecular Approaches to Diagnosing and Managing Infectious Diseases: Practicality and Costs
|Plasmid analysis||Staphylococci Enterobacteriaceae||Plasmids may be digested with restriction endonucleases Only useful when organisms carry plasmids|
|Restriction endonuclease analysis of chromosomal DNA with conventional electrophoresis||Enterococci Staphylococcus aureus Clostridium difficile Candida spp.||Large number of bands Difficult to interpret Not amenable to computer analysis|
|PFGE||Enterobacteriaceae Staphylococci Enterococci Candida spp.||Fewer bands Amenable to computer analysis Very broad application.|
|Genome restriction fragment length polymorphism analysis: ribotyping, insertion sequence probe fingerprinting||Enterobacteriaceae Staphylococci Pseudomonas aeruginosa Mycobacterium tuberculosis Candida spp.||Fewer bands Computer analysis Sequence-based profiles Automated|
|PCR-based methods: repetitive elements PCR spacer typing, selective amplification of genome restriction fragments, multilocus allelic sequence-based typing||Enterobacteriaceae Acinetobacter spp. Staphylococci M. tuberculosis HCV||Crude extracts and small amounts of DNA may suffice|
|Library probe genotypic hybridization schemes: multilocus probe dot-blot patterns, high-density oligonucleotide patterns||Burkholderia cepacia S. aureus M. tuberculosis||Unambiguous yes-no result Less discrimination than other methods Couple with DNA chip technology|
aThe table contains examples of available methods and applications and is not intended to be all-inclusive.
bAdapted from Pfaller (2).