Emerging Infectious Disease ISSN: 1080-6059

Hepatitis E Virus Sequences in Swine Related to Sequences in Humans, the Netherlands

Wim H.M. van der Poel*

, Froukje Verschoor*, Reina van der Heide*, Maria-Inmaculada Herrera†, Amparo Vivo†, Marlou Kooreman*, and Ana Maria de Roda Husman*

Author affiliations: *National Institute of Public Health and the Environment (RIVM), Bilthoven, The Netherlands;; †Instituto de Salud Carlos III, Majadahonda, Madrid, Spain

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Figure 1

Endpoint dilution polymerase chain reaction with the US2 human Hepatitis E virus (HEV) sample as reference (infectivity titer approximately 10 E.6). M: molecular mass marker; lanes 1-4 NLSW50 10-1,10-2,10-3 ;lanes 6-9: NLSW15 10-1,10-2,10-3; lanes 11-14: NLSW20 10-1,10-2,10-3; lanes 16-19 US2 10-1,10-2,10-3. Lanes 5,10 and 15 negative control water samples.

Figure 1. Endpoint dilution polymerase chain reaction with the US2 human Hepatitis E virus (HEV) sample as reference (infectivity titer approximately 10 E.6). M: molecular mass marker; lanes 1-4 NLSW50 10^-1,10^-2,10^-3 ;lanes 6-9: NLSW15 10^-1,10^-2,10^-3; lanes 11-14: NLSW20 10^-1,10^-2,10^-3; lanes 16-19 US2 10^-1,10^-2,10^-3. Lanes 5,10 and 15 negative control water samples.

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