Volume 7, Number 6—December 2001
Research
Hepatitis E Virus Sequences in Swine Related to Sequences in Humans, the Netherlands
Wim H.M. van der Poel*
, Froukje Verschoor*, Reina van der Heide*, Maria-Inmaculada Herrera†, Amparo Vivo†, Marlou Kooreman*, and Ana Maria de Roda Husman*
, Froukje Verschoor*, Reina van der Heide*, Maria-Inmaculada Herrera†, Amparo Vivo†, Marlou Kooreman*, and Ana Maria de Roda Husman*Table 1
Oligonucleotide primers employed for RT-PCR amplification
| Primer | Sense | Sequence (5' to 3') | Position in genome | Reference |
|---|---|---|---|---|
| ORF1-s1 | Sense | Ctggcatyactactgcyattgagc | 56-79 | 13 |
| ORF1-a1 | Antisense | Ccatcrarrcagtaagtgcggtc | 451-473 | 13 |
| ORF1-s2 | Sense | Ctgccytkgcgaatgctgtgg | 104-124 | 12 |
| ORF1-a2 | Antisense | Ggcagwrtaccarcgctgaacatc | 367-389 | 12 |
| ORF2-s1 | Sense | Gacagaattratttcgtcggctgg | 6298-6321 | 13 |
| ORF2-a1 | Antisense | Cttgttcrtgytggttrtcataatc | 6470-6494 | 13 |
| 3156-EF | Sense | Aaytatgcmcagtaccgggttg | 5687-5708 | 7 |
| 3157-ER | Antisense | Cccttatcctgctgagcattctc | 6395-6417 | 7 |
| 3158-EF | Sense | Gtyatgtytytgcatacatggct | 5972-5993 | 7 |
| 3159-IRS | Antisense | Agccgacgaaatyaattctgtc | 6298-6319 | 7 |
aNucleotide positions are numbered according to the Burmese strain (10).
