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Volume 7, Number 6—December 2001


Hepatitis E Virus Sequences in Swine Related to Sequences in Humans, the Netherlands

Wim H.M. van der Poel*Comments to Author , Froukje Verschoor*, Reina van der Heide*, Maria-Inmaculada Herrera†, Amparo Vivo†, Marlou Kooreman*, and Ana Maria de Roda Husman*
Author affiliations: *National Institute of Public Health and the Environment (RIVM), Bilthoven, The Netherlands;; †Instituto de Salud Carlos III, Majadahonda, Madrid, Spain

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Table 1

Oligonucleotide primers employed for RT-PCR amplification

Primer Sense Sequence (5' to 3') Position in genome Reference
ORF1-s1 Sense Ctggcatyactactgcyattgagc 56-79 13
ORF1-a1 Antisense Ccatcrarrcagtaagtgcggtc 451-473 13
ORF1-s2 Sense Ctgccytkgcgaatgctgtgg 104-124 12
ORF1-a2 Antisense Ggcagwrtaccarcgctgaacatc 367-389 12
ORF2-s1 Sense Gacagaattratttcgtcggctgg 6298-6321 13
ORF2-a1 Antisense Cttgttcrtgytggttrtcataatc 6470-6494 13
3156-EF Sense Aaytatgcmcagtaccgggttg 5687-5708 7
3157-ER Antisense Cccttatcctgctgagcattctc 6395-6417 7
3158-EF Sense Gtyatgtytytgcatacatggct 5972-5993 7
3159-IRS Antisense Agccgacgaaatyaattctgtc 6298-6319 7

aNucleotide positions are numbered according to the Burmese strain (10).

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