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Volume 7, Number 6—December 2001

Research

rpoB Gene Mutations in Rifampin-Resistant Mycobacterium tuberculosis Identified by Polymerase Chain Reaction Single-Stranded Conformational Polymorphism

Miriam Bobadilla-del-Valle*, Alfredo Ponce-de-Leon*, Catalina Arenas-Huertero*, Gilberto Vargas-Alarcon†, Midori Kato-Maeda*, Peter M. Small‡, Patricia Couary*, Guillermo M. Ruiz-Palacios*, and Jose Sifuentes-Osornio*Comments to Author 
Author affiliations: *Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubiran, Mexico City, Mexico;; †Instituto Nacional de Cardiología Ignacio Chavez and Universidad Panamericana, Mexico City, Mexico;; ‡Stanford University Medical Center, Stanford, California, USA

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Figure

Representative polymerase chain reaction single-stranded conformational polymorphism (SSCP) patterns of rifampin resistance in Mycobacterium tuberculosis strains. Lanes: 1, 18, molecular weight 174ΦΧHaeIII; lanes: 2, 17, M. tuberculosis rifampin-susceptible control strain H37Rv; lanes 3, 4, 5 (MICs 2,8,64 µg/mL), pattern 1 rifampin-resistant strains with a pattern indistinguishable from that of M. tuberculosis H37Rv; lanes 6,7,8 (MICs 2, 8, 2,048 µg/mL), pattern 2a; lanes 9,10,11 (MICs 2048, 256

Figure. Representative polymerase chain reaction single-stranded conformational polymorphism (SSCP) patterns of rifampin resistance in Mycobacterium tuberculosis strains. Lanes: 1, 18, molecular weight 174ΦΧHaeIII; lanes: 2, 17, M. tuberculosis rifampin-susceptible control strain H37Rv; lanes 3, 4, 5 (MICs 2,8,64 µg/mL), pattern 1 rifampin-resistant strains with a pattern indistinguishable from that of M. tuberculosis H37Rv; lanes 6,7,8 (MICs 2, 8, 2,048 µg/mL), pattern 2a; lanes 9,10,11 (MICs 2048, 256, 256 µg/mL), pattern 2b; lanes 12,13,14,15 (MICs 2048, 2048, 256, 1,024 µg/mL), pattern 2c; lane 16 (MIC 128 µg/mL), pattern 2d.

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