Emerging Infectious Disease ISSN: 1080-6059

rpoB Gene Mutations in Rifampin-Resistant Mycobacterium tuberculosis Identified by Polymerase Chain Reaction Single-Stranded Conformational Polymorphism

Miriam Bobadilla-del-Valle*, Alfredo Ponce-de-Leon*, Catalina Arenas-Huertero*, Gilberto Vargas-Alarcon†, Midori Kato-Maeda*, Peter M. Small‡, Patricia Couary*, Guillermo M. Ruiz-Palacios*, and Jose Sifuentes-Osornio*

Author affiliations: *Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubiran, Mexico City, Mexico;; †Instituto Nacional de Cardiología Ignacio Chavez and Universidad Panamericana, Mexico City, Mexico;; ‡Stanford University Medical Center, Stanford, California, USA

Main Article

Figure

Representative polymerase chain reaction single-stranded conformational polymorphism (SSCP) patterns of rifampin resistance in Mycobacterium tuberculosis strains. Lanes: 1, 18, molecular weight 174ΦΧHaeIII; lanes: 2, 17, M. tuberculosis rifampin-susceptible control strain H37Rv; lanes 3, 4, 5 (MICs 2,8,64 µg/mL), pattern 1 rifampin-resistant strains with a pattern indistinguishable from that of M. tuberculosis H37Rv; lanes 6,7,8 (MICs 2, 8, 2,048 µg/mL), pattern 2a; lanes 9,10,11 (MICs 2048, 256

Figure. Representative polymerase chain reaction single-stranded conformational polymorphism (SSCP) patterns of rifampin resistance in Mycobacterium tuberculosis strains. Lanes: 1, 18, molecular weight 174ΦΧHaeIII; lanes: 2, 17, M. tuberculosis rifampin-susceptible control strain H37Rv; lanes 3, 4, 5 (MICs 2,8,64 µg/mL), pattern 1 rifampin-resistant strains with a pattern indistinguishable from that of M. tuberculosis H37Rv; lanes 6,7,8 (MICs 2, 8, 2,048 µg/mL), pattern 2a; lanes 9,10,11 (MICs 2048, 256, 256 µg/mL), pattern 2b; lanes 12,13,14,15 (MICs 2048, 2048, 256, 1,024 µg/mL), pattern 2c; lane 16 (MIC 128 µg/mL), pattern 2d.

Main Article

Top of Page

The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.