Volume 7, Number 6—December 2001
Research
rpoB Gene Mutations in Rifampin-Resistant Mycobacterium tuberculosis Identified by Polymerase Chain Reaction Single-Stranded Conformational Polymorphism
Miriam Bobadilla-del-Valle*, Alfredo Ponce-de-Leon*, Catalina Arenas-Huertero*, Gilberto Vargas-Alarcon†, Midori Kato-Maeda*, Peter M. Small‡, Patricia Couary*, Guillermo M. Ruiz-Palacios*, and Jose Sifuentes-Osornio*
Table
Mutations of the rpoB gene found in 35 rifampin-resistant Mycobacterium tuberculosis isolates
| Mutated rpoB codon | Specific Mutation | Strain n | MIC (µg/mL) | pc |
|---|---|---|---|---|
| 513 | CAA/AAA(Gln/Lys) | 1 | 2,048 | 0.01 |
| CAA/AAA(Gln/Lys) | 1 | 256 | 0.01 | |
| CAA/CCA(Gln/Pro) | 1 | 2,048 | 0.01 | |
| 516 | GAC/GTC(Asp/Val) | 5 | 37,118 | 0.01, 0.002 |
| 526 | CAC/TAC(His/Tyr) | 1 | 256 | 0.01, 0.002 |
| CAC/TAC(His/Tyr) | 1 | 1,024 | 0.01, 0.002 | |
| CAC/TAC(His/Tyr) | 3 | 2,048 | 0.01, 0.002 | |
| CAC/GAC(His/Asp) | 2 | 256 | 0.01, 0.002 | |
| CAC/GAC(His/Asp) | 1 | 128 | 0.01, 0.002 | |
| CAC/GAC(His/Asp) | 1 | 2,048 | 0.01, 0.002 | |
| 531 | TCG/TTG(Ser/Leu) | 1 | 32 | 0.002 |
| TCG/TTG(Ser/Leu) | 1 | 64 | 0.002 | |
| TCG/TTG(Ser/Leu) | 4 | 256 | 0.002 | |
| TCG/TTG(Ser/Leu) | 1 | 1,024 | 0.002 | |
| TCG/TTG(Ser/Leu) | 3 | 2,048 | 0.002 | |
| TCG/CCG(Ser/Pro)b | 1 | 8 | 0.002 | |
| TCG/GCG(Ser/Ala)b | 1 | 64 | 0.002 | |
| TCG/GCG(Ser/Ala)b | 1 | 128 | 0.002 | |
| TCG/TGG(Ser/Trp) | 1 | 512 | 0.002 | |
| TCG/TTC (Ser/Phe)b | 1 | 2,048 | 0.002 | |
| 522 | TCG/TTG (Ser/Leu) | 1 | 8 | |
| 533 | CTG/CCG (Leu/Pro) | 1 | 2 | |
| 572 | ATC/TTC (Ile/Phe)a | 1 | 2 | |
aThis mutation is located outside the core region.
bNot previously described.
cMann-Whitney test.
