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Volume 7, Number 6—December 2001

Research

rpoB Gene Mutations in Rifampin-Resistant Mycobacterium tuberculosis Identified by Polymerase Chain Reaction Single-Stranded Conformational Polymorphism

Miriam Bobadilla-del-Valle*, Alfredo Ponce-de-Leon*, Catalina Arenas-Huertero*, Gilberto Vargas-Alarcon†, Midori Kato-Maeda*, Peter M. Small‡, Patricia Couary*, Guillermo M. Ruiz-Palacios*, and Jose Sifuentes-Osornio*Comments to Author 
Author affiliations: *Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubiran, Mexico City, Mexico;; †Instituto Nacional de Cardiología Ignacio Chavez and Universidad Panamericana, Mexico City, Mexico;; ‡Stanford University Medical Center, Stanford, California, USA

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Table

Mutations of the rpoB gene found in 35 rifampin-resistant Mycobacterium tuberculosis isolates

Mutated rpoB codon Specific Mutation Strain n MIC (µg/mL) pc
513 CAA/AAA(Gln/Lys) 1 2,048 0.01
CAA/AAA(Gln/Lys) 1 256 0.01
CAA/CCA(Gln/Pro) 1 2,048 0.01
516 GAC/GTC(Asp/Val) 5 37,118 0.01, 0.002
526 CAC/TAC(His/Tyr) 1 256 0.01, 0.002
CAC/TAC(His/Tyr) 1 1,024 0.01, 0.002
CAC/TAC(His/Tyr) 3 2,048 0.01, 0.002
CAC/GAC(His/Asp) 2 256 0.01, 0.002
CAC/GAC(His/Asp) 1 128 0.01, 0.002
CAC/GAC(His/Asp) 1 2,048 0.01, 0.002
531 TCG/TTG(Ser/Leu) 1 32 0.002
TCG/TTG(Ser/Leu) 1 64 0.002
TCG/TTG(Ser/Leu) 4 256 0.002
TCG/TTG(Ser/Leu) 1 1,024 0.002
TCG/TTG(Ser/Leu) 3 2,048 0.002
TCG/CCG(Ser/Pro)b 1 8 0.002
TCG/GCG(Ser/Ala)b 1 64 0.002
TCG/GCG(Ser/Ala)b 1 128 0.002
TCG/TGG(Ser/Trp) 1 512 0.002
TCG/TTC (Ser/Phe)b 1 2,048 0.002
522 TCG/TTG (Ser/Leu) 1 8
533 CTG/CCG (Leu/Pro) 1 2
572 ATC/TTC (Ile/Phe)a 1 2

aThis mutation is located outside the core region.
bNot previously described.
cMann-Whitney test.

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