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Volume 8, Number 1—January 2002

Dispatch

Absence of Mycoplasma Contamination in the Anthrax Vaccine

Mary Kate Hart*, Richard A. Del Giudice†, and George W. Korch*Comments to Author 
Author affiliations: *United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA; †National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland, USA;

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Figure

Evaluation of Anthrax Vaccine Adsorbed (AVA) for amplified mycoplasma DNA by gel electrophoresis. Molecular weight markers were run in lanes 1 and 17. Control samples in lanes 2-5 were mycoplasma broth, Hut 78 cell extract, Acholeplasma laidlawii, and Mycoplasma pirum, respectively. The AVA samples were in lanes 6 to 15: Lot FAV048B from Davis Monthan AFB (lane 6), Kansas City MO NRC (lane 7), and Camp Pendleton (lane 8); Lot FAV047 from Fort Detrick (lanes 9 and 11) and Pearl Harbor NMC (lane 10); Lot FAV031 from Fort Worth Base Naval Clinic (lane 12) and the Pentagon Clinic (lane 13); and Lot FAV008 from Davis Monthan AFB (lane 14) and McEntire ANG Station (lane 15). Lane 16 contained water. Bands seen below 100 base pairs are primer multimers.

Figure. Evaluation of Anthrax Vaccine Adsorbed (AVA) for amplified mycoplasma DNA by gel electrophoresis. Molecular weight markers were run in lanes 1 and 17. Control samples in lanes 2-5 were mycoplasma broth, Hut 78 cell extract, Acholeplasma laidlawii, and Mycoplasma pirum, respectively. The AVA samples were in lanes 6 to 15: Lot FAV048B from Davis Monthan AFB (lane 6), Kansas City MO NRC (lane 7), and Camp Pendleton (lane 8); Lot FAV047 from Fort Detrick (lanes 9 and 11) and Pearl Harbor NMC (lane 10); Lot FAV031 from Fort Worth Base Naval Clinic (lane 12) and the Pentagon Clinic (lane 13); and Lot FAV008 from Davis Monthan AFB (lane 14) and McEntire ANG Station (lane 15). Lane 16 contained water. Bands seen below 100 base pairs are primer multimers.

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