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Volume 8, Number 10—October 2002
Bioterrorism-related Anthrax

Bioterrorism-related Anthrax

Two-Component Direct Fluorescent-Antibody Assay for Rapid Identification of Bacillus anthracis

Barun K. De*Comments to Author , Sandra L. Bragg*, Gary N. Sanden*, Kathy E. Wilson*, Lois A. Diem*, Chung K. Marston*, Alex R. Hoffmaster*, Gwen A. Barnet*, Robbin S. Weyant*, Teresa G. Abshire†, John W. Ezzell†, and Tanja Popovic*
Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA; †U.S. Army Medical Institute of Infectious Diseases, Fort Detrick, Maryland, USA;

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Table 2

Results of cell wall and capsule direct fluorescent-antibody assays for 56 strains of five Bacillus speciesa

Species No. of strains CW-DFA (% positive) CAP-DFA (% positive)
B. cereus 23 10 (43) 0 (0)
B. thuringiensis 12 2 (17) 0 (0)
B. megaterium 11 0 (0) 1 (11)
B. mycoides 1 0 (0) 0 (0)
B. subtilis 9 0 (0) 0 (0)
Total 56 12 (21) 1 (1.7)

aDFA, direct fluorescent-antibody assay; CW, cell wall; CAP, capsule.

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