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Volume 8, Number 10—October 2002
THEME ISSUE
Bioterrorism-related Anthrax

Bioterrorism-related Anthrax

Two-Component Direct Fluorescent-Antibody Assay for Rapid Identification of Bacillus anthracis

Barun K. De*Comments to Author , Sandra L. Bragg*, Gary N. Sanden*, Kathy E. Wilson*, Lois A. Diem*, Chung K. Marston*, Alex R. Hoffmaster*, Gwen A. Barnet*, Robbin S. Weyant*, Teresa G. Abshire†, John W. Ezzell†, and Tanja Popovic*
Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA; †U.S. Army Medical Institute of Infectious Diseases, Fort Detrick, Maryland, USA;

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Table 3

Results of 26 clinical specimens from seven inhalational anthrax patients analyzed by direct fluorescent-antibody assay, culture, and polymerase chain reaction assaya

Patient identifierb Specimen
Results
Type Number Date collected DFA Culture PCRc
1 Heart bloodd,e 1 10/6 (-) ND (-)
1 Bloodd,e 1 10/6 (-) ND (-)
1 Lung tissued,e 2 10/6 (+) ND (-)
1 Chest fluidd,e 2 10/6 (-) ND (+)
1 Pericardial fluidd,e 1 10/6 (-) ND (+)
2 Bloodd 3 10/5 (-) (-) (-)
2 Pleural fluidd 1 10/5 (+) (-) (+)
2 Pleural fluidd 1 10/5 (-) (-) (+)
2 Unspecified body fluidd 1 10/5 (+) (-) (-)
3 Bloodd 1 10/19 (+) (-) (+)
5 Bloodd 2 10/21 (+) (+) (+)
6 Blood 1 10/22 (+) (+) (+)
10 Lung tissued,e 1 10/31 (-) (-) (+)
10 Lymph noded,e 1 10/31 (-) (-) (+)
10 Pleural fluidd 1 10/29 (+) (-) (+)
10 Pleural fluidd 1 10/29 (-) (-) (+)
11 Blood 2 11/17 (+) (+) ND
11 Blood 2 11/17 (+) (+) ND
11 Lymph noded-f 1 11/21 (+) (-) (+)

aDFA, direct fluorescent-antibody assay; PCR, polymerase chain reaction; ND, not done.
bPatients 1, 2, 3, 5, 6, 10 reported by Jernigan et al. (6), and patient 11 reported by Barakat et al. (18).
cReal-time PCR as described by Hoffmaster et al. (20). All DNA samples tested positive by human beta actin PCR.
dSpecimens collected the day on or after antimicrobial treatment was begun.
eSpecimens collected postmortem.
fDocumented culture negative; previously reported as culture positive (18).

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