Evaluation and Validation of a Real-Time Polymerase Chain Reaction Assay for Rapid Identification of Bacillus anthracis
Alex R. Hoffmaster, Richard F. Meyer, Michael P. Bowen, Chung K. Marston, Robbin S. Weyant, Kathy Thurman, Sharon L. Messenger, Erin E. Minor, Jonas M. Winchell, Max V. Rasmussen, Bruce R. Newton, J. Todd Parker, William E. Morrill, Nancy McKinney, Gwen A. Barnett, James J. Sejvar, John A. Jernigan, Bradley A. Perkins, and Tanja Popovic
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA;
Figure. Real-time polymerase chain reaction graph of three B. anthracis markers and B-actin control detected in a pleural fluid specimen from a patient with inhalational anthrax. The horizontal line indicates a threshold value; the vertical lines indicate cross-threshold values for each marker. BA1, primer and probe set targeting a region of pXO2; BA2, primer and probe set targeting a region of pXO1; BA3, primer/probe set targeting a region of B. anthracis chromosome.
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