Emerging Infectious Disease ISSN: 1080-6059

Evaluation and Validation of a Real-Time Polymerase Chain Reaction Assay for Rapid Identification of Bacillus anthracis

Alex R. Hoffmaster, Richard F. Meyer, Michael P. Bowen, Chung K. Marston, Robbin S. Weyant, Kathy Thurman, Sharon L. Messenger, Erin E. Minor, Jonas M. Winchell, Max V. Rasmussen, Bruce R. Newton, J. Todd Parker, William E. Morrill, Nancy McKinney, Gwen A. Barnett, James J. Sejvar, John A. Jernigan, Bradley A. Perkins, and Tanja Popovic

Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA;

Main Article

Figure

Real-time polymerase chain reaction graph of three B. anthracis markers and B-actin control detected in a pleural fluid specimen from a patient with inhalational anthrax. The horizontal line indicates a threshold value; the vertical lines indicate cross-threshold values for each marker. BA1, primer and probe set targeting a region of pXO2; BA2, primer and probe set targeting a region of pXO1; BA3, primer/probe set targeting a region of B. anthracis chromosome.

Figure. Real-time polymerase chain reaction graph of three B. anthracis markers and B-actin control detected in a pleural fluid specimen from a patient with inhalational anthrax. The horizontal line indicates a threshold value; the vertical lines indicate cross-threshold values for each marker. BA1, primer and probe set targeting a region of pXO2; BA2, primer and probe set targeting a region of pXO1; BA3, primer/probe set targeting a region of B. anthracis chromosome.

Main Article

Top of Page

The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.