Volume 8, Number 10—October 2002
Letter
Evaluation and Validation of a Real-Time Polymerase Chain Reaction Assay for Rapid Identification of Bacillus anthracis
Table
Origin, designations, and results of real-time polymerase chain reaction assay for Bacillus anthracis strainsa
| No. positive/total | ||||||
|---|---|---|---|---|---|---|
| B. anthracis | No. analyzed | Temporal range and geographic origin | MLVA genotypes representeda | Ba1b | Ba2b | Ba3b |
| Human isolates | 30 | 1943–1996 Africa, Asia, Australia, Europe, North America | 3, 4, 22, 23, 28, 32, 34, 35, 36, 37, 41, 43, 44, 45, 50, 66, 68 | 30/30 | 30/30 | 30/30 |
| Animal isolates | 29 | Africa, Asia, Australia, Europe, North America, South America | 3, 10, 20, 26, 29, 30, 35, 38, 40, 45, 48, 49, 51, 55, 57, 78, 80, 81, 84, 85, 87, 89 | 29/29 | 29/29 | 29/29 |
| Other isolates | 16 | 1950–1993 Africa, Asia, Europe, N. America | 13, 14, 21, 24, 47, 62, 69, 73, 77, 79, 82 | 16/16 | 16/16 | 16/16 |
| Outbreak isolates | 317 | 2001 U.S. outbreak | 62 | 317/317 | 317/317 | 317/317 |
| pXO1 cured | 5 | 1956–1974 North America | 5/5 | 0/5 | 5/5 | |
| pXO2 cured | 1 | Africa | 0/1 | 1/1 | 1/1 | |
aMLVA, multiple-locus variable-number tandem repeat analysis as described by Keim et al. (15).
bBa1, Ba2, and Ba3 primer/probe sets as described in Materials and Methods.
