Volume 8, Number 10—October 2002
Evaluation and Validation of a Real-Time Polymerase Chain Reaction Assay for Rapid Identification of Bacillus anthracis Supplement
|Patient no.c||Laboratory confirmation||Other laboratory tests positive for Bacillus anthracis|
|1||CSF culture||IHC of multiple (postmortem) tissues, blood culture|
|2||PCR of pleural fluid; IHC of pleural fluid; serology||Transbronchial biopsy IHC, pleural biopsy IHC|
|3||Blood culture||PCR of blood; serology|
|5||Blood culture||IHC of mediastinal lymph nodes; PCR of blood|
|6||Blood culture||IHC of mediastinal lymph nodes; PCR of blood|
|8||PCR of pleural fluid; IHC of pleural fluid||Serology|
|9||IHC of pleural fluid and bronchial biopsy; serology|
|10||Blood and pleural fluid culture||IHC of multiple organs; PCR of multiple organs|
|11||Blood culture||PCR of multiple organs; IHC of multiple organs|
aAll initial isolation of Bacillus anthracis from clinical specimens took place at the local health facility where the patients were treated.
bCSF, cerebrospinal fluid; IHC, immunohistochemical stain; PCR, polymerase chain reaction.
cPatients 1–10 described in Jernigan et al. (1) and patient 11 in Barakat et al. (5).