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Volume 8, Number 10—October 2002


Evaluation and Validation of a Real-Time Polymerase Chain Reaction Assay for Rapid Identification of Bacillus anthracis Supplement

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Table 1

Laboratory methods used for confirmation of 11 inhalational anthrax casesa,b

Patient no.c Laboratory confirmation Other laboratory tests positive for Bacillus anthracis
1 CSF culture IHC of multiple (postmortem) tissues, blood culture
2 PCR of pleural fluid; IHC of pleural fluid; serology Transbronchial biopsy IHC, pleural biopsy IHC
3 Blood culture PCR of blood; serology
4 Blood culture Serology
5 Blood culture IHC of mediastinal lymph nodes; PCR of blood
6 Blood culture IHC of mediastinal lymph nodes; PCR of blood
7 Blood culture Serology
8 PCR of pleural fluid; IHC of pleural fluid Serology
9 IHC of pleural fluid and bronchial biopsy; serology
10 Blood and pleural fluid culture IHC of multiple organs; PCR of multiple organs
11 Blood culture PCR of multiple organs; IHC of multiple organs

aAll initial isolation of Bacillus anthracis from clinical specimens took place at the local health facility where the patients were treated.
bCSF, cerebrospinal fluid; IHC, immunohistochemical stain; PCR, polymerase chain reaction.
cPatients 1–10 described in Jernigan et al. (1) and patient 11 in Barakat et al. (5).

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