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Volume 8, Number 10—October 2002

Letter

Evaluation and Validation of a Real-Time Polymerase Chain Reaction Assay for Rapid Identification of Bacillus anthracis Supplement

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Table 3

Results of real-time PCR and culture testing performed on 382 clinical specimensa,b


PCR only
PCR and culture
Total
Nine inhalational cases + - PCR + C - PCR + C + PCR - C - PCR - C +
Blood specimens
5
20
9
5
35
0
74
Swab specimens
0
0
0
0
0
0
0
Serum specimens
2
15
3
0
16
0
36
Sputum specimens
0
1
1
0
0
0
2
Tissue specimens
0
1
3
0
3
0
7
Pleural fluid







specimens
5
0
11
0
3
0
19
Other specimens
1
0
2
0
1
0
4
Totals
50
92
142




Seven cutaneous cases







Blood specimens
0
0
1
0
10
0
11
Swab specimens
0
0
0
0
3
0
3
Serum specimens
0
2
0
0
11
0
13
Sputum specimens
0
0
0
0
0
0
0
Tissue specimens
0
2
2
0
4
0
8
Other specimens
0
0
0
0
2
0
2
Totals
4
33
37




Four suspect cases







Blood specimens
0
2
0
0
7
0
9
Swab specimens
0
0
0
0
2
0
2
Serum specimens
0
1
0
0
3
0
4
Sputum specimens
0
0
0
0
0
0
0
Tissue specimens
0
2
0
0
0
0
2
 Other specimens
0
0
0
0
0
0
0
Totals
5
12
17




Other







Blood specimens
0
16
0
0
58
0
74
Swab specimens
0
1
0
0
14
0
15
Serum specimens
0
11
0
0
30
0
41
Sputum specimens
0
0
0
0
4
0
4
Tissue specimens
0
14
0
0
24
0
38
Other specimens 0 2 0 0 12 0 14
Totals 44 142 186

aSixteen patients with laboratory-confirmed anthrax, four suspect cases of anthrax, and 74 patients on whom anthrax has been ruled out.
bPCR, polymerase chain reaction; C, culture.

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