Molecular Analysis of Sarcoidosis Tissues for Mycobacterium Species DNA
Wonder Puryear Drake* , Zhiheng Pei†, David T. Pride†, Robert D. Collins*, Timothy L. Cover*, and Martin J. Blaser†
Author affiliations: *Vanderbilt University School of Medicine and Veterans Affairs Medical Center, Nashville, Tennessee, USA; †New York University School of Medicine and Veterans Affairs Medical Center, New York, New York, USA;
Figure. Analysis of 16S rRNA sequences from nine Mycobacterium species and from 12 patients with sarcoidosis. Phylograms based on nucleotide alignments were generated with HKY85 distances matrices using Paup 4.0b10 (Sinauer Associates, Sunderland MA). Bootstrap values >50 (based on 500 replicates) are represented at each node, and the branch length index is represented below the phylogram. For eight patients, the 16S rRNA sequence was identical to M. tuberculosis, and for one patient (patient 24) was identical to M. gordonae. For patient 15, the sequence was closely related to M. tuberculosis; for patient 19, the sequence was closely related to M. duvalii and M. gordonae; for patient 7, the sequence was less closely related to M. leprae.
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