Figure 2. Phylogenetic tree of nucleotide sequences of 163-bp Orthopoxvirus fusion gene amplicons from the patient and rat (head and paw), Cowpox virus, Vaccinia virus (strain Ankara and WR), Camelpox virus, Monkeypox virus, Variola virus, and Ectromelia virus (Mousepox virus). The nucleotide sequences were aligned by using BioEdit software package (T. Hall, Dept. of Microbiology, Raleigh, NC). Phylogenetic relationships were determined by using the Lasergene software packages (DNASTAR Inc., Madison, WI).