Emerging Infectious Disease ISSN: 1080-6059

European Bat Lyssavirus Infection in Spanish Bat Populations

Jordi Serra-Cobo*

, Blanca Amengual†, Carlos Abellán‡, and Hervé Bourhy†

Author affiliations: *Universitat de Barcelona, Barcelona, Spain; †Institut Pasteur, Paris, France; ‡Ministerio de Sanidad y Consumo, Madrid, Spain;

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Figure 2

Detection of European bat Lyssavirus 1 RNA in bats by nested reverse transcription-polymerase chain reaction (PCR). Lanes: 1, brain of Miniopterus schreibersii No. 140; 2- 5, medulla, tongue, esophagus-larynx-pharynx, and lung of Rhinolophus ferrumequinum No. 135, respectively; 6-14, brain, medulla, esophagus-larynx-pharynx, liver, lung, heart, tongue, spleen, and kidney of R. ferrumequinum No. 128, respectively; 15, negative control of RNA extraction of bat No. 135; 16, negative control of RNA extraction of bat No. 128; 17, negative control of RNA extraction of bat No. 140; 18, positive control; 19, negative control of first PCR; 20, negative control of second PCR.

Figure 2. Detection of European bat Lyssavirus 1 RNA in bats by nested reverse transcription-polymerase chain reaction (PCR). Lanes: 1, brain of Miniopterus schreibersii No. 140; 2- 5, medulla, tongue, esophagus-larynx-pharynx, and lung of Rhinolophus ferrumequinum No. 135, respectively; 6-14, brain, medulla, esophagus-larynx-pharynx, liver, lung, heart, tongue, spleen, and kidney of R. ferrumequinum No. 128, respectively; 15, negative control of RNA extraction of bat No. 135; 16, negative control of RNA extraction of bat No. 128; 17, negative control of RNA extraction of bat No. 140; 18, positive control; 19, negative control of first PCR; 20, negative control of second PCR.

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