Figure 2. Detection of European bat Lyssavirus 1 RNA in bats by nested reverse transcription-polymerase chain reaction (PCR). Lanes: 1, brain of Miniopterus schreibersii No. 140; 2- 5, medulla, tongue, esophagus-larynx-pharynx, and lung of Rhinolophus ferrumequinum No. 135, respectively; 6-14, brain, medulla, esophagus-larynx-pharynx, liver, lung, heart, tongue, spleen, and kidney of R. ferrumequinum No. 128, respectively; 15, negative control of RNA extraction of bat No. 135; 16, negative control of RNA extraction of bat No. 128; 17, negative control of RNA extraction of bat No. 140; 18, positive control; 19, negative control of first PCR; 20, negative control of second PCR.
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