Figure 2. Analysis of flagella purified from SMDP92. (A) sodium dodecyl-sulfate polyacrylamide gel electrophoresis of purified flagella, showing the 38-kDa flagellin subunit. Lane 1, molecular weight standards; lane 2, purified SMFliC. (B) Immunoblotting and reactivity of purified flagella with anti-SMFliC antibodies. The 38-kDa flagellin is indicated by an arrow. (C) Electron microscopy of purified flagella visualized by negative staining. Bar, 0.37 μm.