Characterization of Flagella Produced by Clinical Strains of Stenotrophomonas maltophilia
Doroti de Oliveira-Garcia*†, Monique Dall'Agnol‡, Mónica Rosales‡, Ana C.G.S. Azzuz§, Marina B. Martinez†§, and Jorge A. Girón‡
Author affiliations: *Laboratório Clínico do Instituto Dante Pazzanese de Cardiologia, São Paulo, Brazil; †Departamento de Microbiologia do Instituto de Ciências Biomédicas da Universidade de São Paulo, São Paulo, Brazil; ‡Benemerita Universidad Autónoma de Puebla, Puebla, México; §Departamento de Análises Clínicas da Faculdade de Ciências Farmacêuticas da Universidade de São Paulo, São Paulo, Brazil;
Figure 2. Analysis of flagella purified from SMDP92. (A) sodium dodecyl-sulfate polyacrylamide gel electrophoresis of purified flagella, showing the 38-kDa flagellin subunit. Lane 1, molecular weight standards; lane 2, purified SMFliC. (B) Immunoblotting and reactivity of purified flagella with anti-SMFliC antibodies. The 38-kDa flagellin is indicated by an arrow. (C) Electron microscopy of purified flagella visualized by negative staining. Bar, 0.37 μm.
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