Volume 8, Number 9—September 2002
Dispatch
Detection by Enzyme-Linked Immunosorbent Assay of Antibodies to West Nile virus in Birds
Gregory D. Ebel*
, Alan P. Dupuis*, David Nicholas*, Donna Young*, Joseph Maffei*, and Laura D. Kramer*
, Alan P. Dupuis*, David Nicholas*, Donna Young*, Joseph Maffei*, and Laura D. Kramer*Table
Comparison of serologic assay results with reactivity across a range of avian orders and species
| Common Name | Species | Order | Indirect ELISA P/Na | PRNT titer |
|---|---|---|---|---|
| House Sparrow # 1 | Passer domesticus | Passeriformes | 3.0 | 40 |
| House Sparrow # 2 | P. domesticus | Passeriformes | 3.0 | 20 |
| House Sparrow # 3 | P. domesticus | Passeriformes | 7.2 | 80 |
| Northern Mockingbird | Mimus polyglottos | Passeriformes | 2.1 | >10 |
| European Starling | Sturnus vulgaris | Passeriformes | 3.0 | 40 |
| American Crow #1 | Corvus brachyrhynchos | Passeriformes | 2.8 | 80 |
| American Crow #2 | C. brachyrhynchos | Passeriformes | 2.0 | 20 |
| Rock Dove #1 | Columba livia | Columbiformes | 2.9 | 80 |
| Rock Dove #2 | C. livia | Columbiformes | 2.5 | 320 |
| Rock Dove #3 | C. livia | Columbiformes | 7.6 | 10 |
| White-naped Crane | Grus vipio | Gruiformes | 5.3 | >640 |
| Waldrapp Ibis #1 | Gerontica eremita | Ciconiiformes | 9.4 | 320 |
| Waldrapp Ibis #2 | G. eremita | Ciconiiformes | 11.4 | 640 |
| Waldrapp Ibis #3 | G. eremita | Ciconiiformes | 4.0 | 80 |
| Black Crowned Night Heron | Nycticorax nycticorax | Ciconiiformes | 5.2 | 160 |
| Flamingo #1 | Phoenicopterus chilensis | Phoenicopteriformes | 3.7 | 160 |
| Flamingo #2 | P. chilensis | Phoenicopteriformes | 4.2 | >640 |
| Malay Great Argus | Argusianus argus | Galliformes | 7.3 | 40 |
| Kenya Crested Guineafowl | Guttera edouardi | Galliformes | 6.7 | 80 |
| Wild Turkey | Meleagris gallopavo | Galliformes | 6.0 | 320 |
| Bulwer’s Pheasant | Lophura bulweri | Galliformes | 3.5 | >640 |
| American White Pelican | Pelecanus erythrorhynchos | Pelecaniformes | 5.7 | >640 |
| Guanay Cormorant | Phalacrocorax bougainvillii | Pelecaniformes | 2.1 | 80 |
| Brown Pelican | Pelecanus occidentalis | Pelecaniformes | 3.8 | 160 |
| Domestic Goose | Anser sp. | Anseriformes | 3.6 | 40 |
| Trumpeter Swan | Cygnus buccinator | Anseriformes | 4.0 | 160 |
| Barred Owl | Strix varia | Strigiformes | 2.6 | 160 |
| Ostrich | Struthio camelus | Struthioniformes | 3.9 | 640 |
| Magellanic Penguin | Spheniscus magellanicus | Sphenisciformes | 4.3 | >640 |
| Black-Necked Crane | Grus nigricollis | Gruiformes | 2.4 | 80 |
| Laughing Gull |
Larus atricilla |
Charadriiformes |
6.9 |
320 |
| Domestic Duck | Anas sp. | Anseriformes | 1.1 | <10 |
| Canada Goose | Branta canadensis | Anseriformes | 1.2 | <20 |
| Domestic chicken | Gallus gallus | Galliformes | 0.9 | <10 |
| SLEV-positive chicken | G. gallus | Galliformes | 2.5 | 20 |
| WEEV-positive chicken | G. gallus | Galliformes | 1.3 | <20 |
WNV-negative specimens shown below bold line
aELISA, enzyme-linked immunosorbent assay; P/N, positive/negative ratio; PRNT, plaque-reduction neutralization tests; SLEV, St. Louis encephalitis virus; WEEV, Western equine encephalomyelitis virus.
