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Volume 9, Number 12—December 2003

Research

The Rabbit as a New Reservoir Host of Enterohemorrhagic Escherichia coli

Alexis García*Comments to Author  and James G. Fox*
Author affiliations: *Massachusetts Institute of Technology, Cambridge, Massachusetts, USA

Main Article

Figure 1

Southern blot analysis of DNA from rabbit Escherichia coli isolates by using a Shiga toxin 1B (stx1B) probe from rabbit enterohemorrhagic E. coli (EHEC) O153:H-. Lane 1, E. coli O157:H7 DNA (EDL 933, human isolate, positive control); lane 2, No DNA; lanes 3–5, O153:H- DNA (rabbit isolates 01-3014, 02-3283, 02-3300, respectively); lanes 6 and 7, O153:H7 DNA (02-3446 and 02-3301); lanes 8 and 9, O145:H- DNA (02-3282 and 02-3055); lane 10, rabbit isolate of unknown O serotype (03-192); lane 11, O14

Figure 1. Southern blot analysis of DNA from rabbit Escherichia coli isolates by using a Shiga toxin 1B (stx1B) probe from rabbit enterohemorrhagic E. coli (EHEC) O153:H-. Lane 1, E. coli O157:H7 DNA (EDL 933, human isolate, positive control); lane 2, No DNA; lanes 3–5, O153:H- DNA (rabbit isolates 01-3014, 02-3283, 02-3300, respectively); lanes 6 and 7, O153:H7 DNA (02-3446 and 02-3301); lanes 8 and 9, O145:H- DNA (02-3282 and 02-3055); lane 10, rabbit isolate of unknown O serotype (03-192); lane 11, O145:H7 DNA (02-3448). E. coli 02-3300 and 02-3301 were eae- and were isolated from a New Zealand White rabbit. E. coli 02-3055 and 03-192 were both isolated from Dutch Belted rabbit 02-177.

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