Araçatuba Virus: A Vaccinialike Virus Associated with Infection in Humans and Cattle
Giliane de Souza Trindade*, Flávio Guimarães da Fonseca†, João Trindade Marques*, Maurício Lacerda Nogueira†, Luiz Claudio Nogueira Mendes‡, Alexandre Secorun Borges‡§, Juliana Regina Peiró‡, Edviges Maristela Pituco¶, Cláudio Antônio Bonjardim*, Paulo César Peregrino Ferreira*, and Erna Geessien Kroon*
Author affiliations: *Universidade Federal de Minas Gerais, Belo Horizonte, Brasil; †National Institutes of Health, Bethesda, Maryland, USA; ‡Universidade Estadual Paulista–Araçatuba, São Paulo, Brasil; §Universidade Estadual Paulista–Botucatu, São Paulo, Brasil; ¶Instituto Biológico, São Paulo, Brasil
Figure 3. Detection and restriction fragment length polymorphism taxonomic analysis of the Araçatuba virus ATI gene. Primers based on the ATI gene nucleotide sequence from the cowpox virus were used to amplify the gene. (A) The amplified fragments were resolved on 0.6% agarose gel with ethidium bromide. Line 1 shows Araçatuba virus; line 2 shows vaccinia virus; and line 3 shows cowpox virus, Brighton strain. (B) Products obtained after amplification were digested with XbaI restriction enzyme. Fragments were resolved on 1.5% agarose gel stained with ethidium bromide. Arrowheads indicate molecular sizes (line 1, Araçatuba virus; line 2, vaccinia virus; line 3, cowpox virus, Brighton strain.
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