Figure 3. Detection and restriction fragment length polymorphism taxonomic analysis of the Araçatuba virus ATI gene. Primers based on the ATI gene nucleotide sequence from the cowpox virus were used to amplify the gene. (A) The amplified fragments were resolved on 0.6% agarose gel with ethidium bromide. Line 1 shows Araçatuba virus; line 2 shows vaccinia virus; and line 3 shows cowpox virus, Brighton strain. (B) Products obtained after amplification were digested with XbaI restriction enzyme. Fragments were resolved on 1.5% agarose gel stained with ethidium bromide. Arrowheads indicate molecular sizes (line 1, Araçatuba virus; line 2, vaccinia virus; line 3, cowpox virus, Brighton strain.