Figure 3. EcoRI restriction patterns of plasmids from Escherichia coli transconjugants of clinical isolates (A and C) with a CMY-2-like enzyme and blaCMY Southern hybridization (B and D). Lanes 1–19, restriction profiles (TP1–TP19) of plasmids from 19 transconjugants of E. coli isolates; lanes 20–21, transconjugants of Salmonella isolates ST275/00 and ST595/00; lanes 22–23, transconjugants of K. pneumoniae isolates KP218/00 and KP1905/00; lane 24, transconjugant of E. coli isolate EC811/00; lanes M1 and M2, 1-kb molecular marker and molecular marker II, respectively. Arrows indicate the locations of restriction fragments that were hybridized with the blaCMY probe.
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