Emerging Infectious Disease ISSN: 1080-6059

Bartonella henselae in Ixodes ricinus Ticks (Acari: Ixodida) Removed from Humans, Belluno Province, Italy

Yibayiri O. Sanogo*, Zaher Zeaiter*, Guiseppe Caruso†, Francesco Merola†, Stanislav Shpynov*, Philippe Brouqui*, and Didier Raoult*

Author affiliations: *Faculté de Médecine, Marseille, France; †Ospedale San Martino, Belluno, Italy

Primers used for polymerase chain reaction or sequencing

Primer	Bartonella species	Primer sequence	Target gene	References
HSPF1d^a,b	All	5′-GAACTNGAAGATAAGTTNGAA-3′	groEL	22
BbHS1630.n^a,b	All	5′-AATCCATTCCGCCCATTC-3′	groEL	18
HSP1 ^b	All	5′-GGAAAAAGTNGGCAATGAAG-3'	groEL	22
HSP2^b	All	5′-GCNGCTTCTTCACCNGCATT-3′	groEL	22
HSPS1^b	All	5′-AAGCNCCNGGNTTTGGTGA-3′	groEL	22
HSPS2^b	All	5′-TCACCAAANCCNGGNGCTT-3′	groEL	22
HSPF2d^b	All	5′-GAAAGANCGNGTNGATGAT-3'	groEL	22
HSPR2d^b	All	5′-GTNATNAGAAGNCTNGCAAT-3′	groEL	22
PAPn1^a,b	B. henselae and B. quintana	5′-TTCTAGGAGTTGAAACCGAT-3′	pap31	22
PAPn2^a,b	B. henselae and B. quintana	5′-GAAACACCACCAGCAACATA-3′	pap31	22
PAPns2^a,b	B. henselae and B. quintana	5′-GCACCAGACCATTTTTCCTT-3'	pap31	22
PAPns1^b	B. henselae and B. quintana	5′-CAGAGAAGACGCAAAAACCT-3'	pap31	22
BaftsZF	B. henselae	5′-GCTAATCGTATTCGCGAAGAA-3′	ftsZ	This study
BaftsZR	B. henselae	5′-GCTGGTATTTCCAAYTGATCT-3′	ftsZ	This study
BhftsZ 1393.n	B. henselae	5′-GCGAACTACGGCTTACTTGC-3′	ftsZ	21
Bh ftsZ 1247.p	B. henselae	5′-CGGTTGGAGAGCAGTTTCGTC-3′	ftsZ	21

^aAmplification primer.
^bSequencing primer.