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Volume 9, Number 3—March 2003

Research

Molecular Detection of Bartonella quintana, B. koehlerae, B. henselae, B. clarridgeiae, Rickettsia felis, and Wolbachia pipientis in Cat Fleas, France

Jean-Marc Rolain*, Michel Franc†, Bernard Davoust‡, and Didier Raoult*Comments to Author 
Author affiliations: *Université de la Méditerranée, Marseille, France; †Ecole Nationale Vétérinaire de Toulouse, France; ‡Conseiller Vétérinaire Régional Interarmées, Lyon Armées, France)

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Table 1

Oligonucleotide primers used for polymerase chain reaction amplification and sequencing

Primer (reference) Nucleotide sequence Detected organism References
CS-877 (gltA gene) GGG GGC CTG CTC ACG GCG G Rickettsia species (17)
CS-1273 (gltA gene)
BM59 (rOmpB gene)
B807 (rOmpB gene) ATT GCA AAA AGT ACA GTG AAC A
CCG CAG GGT TGG TAA CTG C
CCT TTT AGA TTA CCG CCT AA Rickettsia species
Rickettsia species
Rickettsia species (17)
(18)
(18)
URBarto1 (its gene) CTT CGT TTC TCT TTC TTC A Bartonella species (19)
URBarto2 (its gene) CTT CTC TTC ACA ATT TCA AT Bartonella species (19)
PAPn1 (pap31 gene) TTC TAG GAG TTG AAA CCG AT Bartonella species (20)
PAPn2 (pap31 gene) GAA ACA CCA CCA GCA ACA TA Bartonella species (20)
BartogltAForward (gltA gene) TTC CGY CTT ATG GGT TTT GG Bartonella species This report
Bartokoehlerae (gltA gene) AAC AAA ATA TTC ATC ATT CAG G B. koehlerae This report
Bartoclarridgeiae (gltA gene) AAA GCA ATT TTT TCA AGT TCC B. clarridgeiae This report
Bartohenselae (gltA gene) CAT TTC TGT TGG AAA TCC TAG B. henselae This report
Bartoquintana (gltA gene)
EHR16SD (16S rRNA gene)
EHR16SD (16S rRNA gene) TTT TAA TGT AAT GCC AGA ATA A
GGT ACC YAC AGA AGA AGT CC
TAG CAC TCA TCG TTT ACA GC B. quintanaWolbachiaWolbachia This report
(21)
(21)

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