Volume 9, Number 5—May 2003
Research
Variant Salmonella Genomic Island 1 Antibiotic Resistance Gene Cluster in Salmonella enterica Serovar Albany
Benoît Doublet*, Renaud Lailler†, Danièle Meunier*, Anne Brisabois†, David Boyd‡, Michael R. Mulvey‡, Elisabeth Chaslus-Dancla*, and Axel Cloeckaert*
Table
Primers used for polymerase chain reaction
| Primer | Gene | Amplificationa | Size (bp) | Nucleotide sequence (5´–3´) |
|---|---|---|---|---|
| U7-L12 |
thdf |
Left junction |
500 |
ACACCTTGAGCAGGGCAAG |
| LJ-R1 |
int |
AGTTCTAAAGGTTCGTAGTCG |
||
| 104-RJ |
S044 |
Right junction |
TGACGAGCTGAAGCGAATTG |
|
| C9-L2 |
int2 |
515 |
AGCAAGTGTGCGTAATTTGG |
|
| 104-D |
yidY |
500 |
ACCAGGGCAAAACTACACAG |
|
| cml01 |
floR |
floR |
494 |
TTTGGWCCGCTMTCRGAC |
| cml15 |
floR |
SGAGAARAAGACGAAGAAG |
||
| int1 |
intI1 |
A |
1,135 |
GCTCTCGGGTAACATCAAGG |
| aad |
aadA2 |
GACCTACCAAGGCAACGCTA |
||
| sulTER |
sul1delta |
B |
942 |
AAGGATTTCCTGACCCTG |
| F3 |
floR |
AAAGGAGCCATCAGCAGCAG |
||
| F4 |
floR |
C |
598 |
TTCCTCACCTTCATCCTACC |
| F6 |
tetR |
TTGGAACAGACGGCATGG |
||
| tetR |
tetR |
D |
1,559 |
GCCGTCCCGATAAGAGAGCA |
| tetA |
tetA |
GAAGTTGCGAATGGTCTGCG |
||
| int2 |
groEL-intI1 |
E |
1,338 |
TTCTGGTCTTCGTTGATGCC |
| pse1 |
pse-1 |
CATCATTTCGCTCTGCCATT |
||
| pse-L |
pse-1 |
F |
4,400 |
AATGGCAATCAGCGCTTCCC |
| MDR-B | S044 | GAATCCGACAGCCAACGTTCC |
aSee Figure 1.
