Volume 9, Number 7—July 2003
Dispatch
Serologic Evidence of West Nile Virus Infection in Horses, Coahuila State, Mexico
Bradley J. Blitvich*, Ildefonso Fernandez-Salas†, Juan F. Contreras-Cordero†, Nicole L. Marlenee*, Jose I. Gonzalez-Rojas†, Nicholas Komar‡, Duane J. Gubler‡, Charles H. Calisher*, and Barry J. Beaty*
Table
Summary of serologic data for horses in Coahuila State, Mexicoa
| Horse | Study site | Clinical illness | % inhibition by ELISAb | cPRNT90 titer |
PRNT diagnosis | |
|---|---|---|---|---|---|---|
| WNV | SLEV | |||||
| H-1 |
Ciudad Acuña |
No |
90 |
>320 |
—d |
WNV |
| H-2 |
Ciudad Acuña |
No |
5 |
— |
— |
Negative |
| H-3 |
Ciudad Acuña |
No |
0 |
— |
— |
Negative |
| H-4 |
Ciudad Acuña |
No |
0 |
— |
— |
Negative |
| H-5 |
Ciudad Acuña |
No |
90 |
>320 |
40 |
WNV |
| H-6 |
Ciudad Acuña |
No |
93 |
>320 |
— |
WNV |
| H-7 |
Ciudad Acuña |
No |
93 |
>320 |
— |
WNV |
| H-8 |
Ciudad Acuña |
Yes |
93 |
>320 |
— |
WNV |
| H-9 |
Ciudad Acuña |
Yes |
86 |
>320 |
— |
WNV |
| H-10 |
Ciudad Acuña |
Yes |
90 |
>320 |
20 |
WNV |
| H-11 |
Ciudad Acuña |
No |
89 |
>320 |
— |
WNV |
| H-12 |
Ciudad Acuña |
No |
92 |
>320 |
20 |
WNV |
| H-13 |
Ciudad Acuña |
Yes |
91 |
>320 |
— |
WNV |
| H-14 |
Ciudad Acuña |
Yes |
78 |
>320 |
20 |
WNV |
| H-15 |
Jiménez |
No |
82 |
>320 |
— |
WNV |
| H-16 |
Jiménez |
No |
25 |
40 |
— |
WNV |
| H-17 |
Jiménez |
No |
7 |
— |
— |
Negative |
| H-18 |
Jiménez |
No |
93 |
>320 |
20 |
WNV |
| H-19 |
Jiménez |
No |
0 |
20 |
20 |
Flavivirus |
| H-20 |
Jiménez |
No |
47 |
40 |
— |
WNV |
| H-21 |
Saltillo |
No |
9 |
— |
— |
Negative |
| H-22 |
Saltillo |
No |
15 |
— |
— |
Negative |
| H-23 |
Saltillo |
No |
12 |
— |
— |
Negative |
| H-24 | Saltillo | No | 11 | — | — | Negative |
aELISA, enzyme-linked immunosorbent assay; PRNT, plaque reduction neutralization test; WNV, West Nile virus; SLEV, Saint Louis encephalitis virus.
bInhibition values >30% are considered significant.
cNeutralizing antibodies to WNV in selected horse serum samples were confirmed at Centers for Disease Control and Prevention-Division of Viral and Bacterial Infectious Diseases by PRNT.
d—, <20.
