Figure 2. Phylogenetic tree of the VP1 3′ terminal region, which identifies the Spanish isolates as echovirus 13 (EV13) and differentiates two clusters (Iberian Peninsula and Canary Islands). The sequences included are, apart from the reference strains, all the Spanish EV13 (61 isolates and 3 sequences obtained directly from cerebrospinal fluid) and two American isolates. Model of nucleotide substitution: Kimura two parameters. Phylogenetic tree reconstructed with the neighbor joining method, and bootstrap analysis with 1,000 pseudoreplicate datasets.
The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.