Figure 2. Phylogenetic tree of the VP1 3′ terminal region, which identifies the Spanish isolates as echovirus 13 (EV13) and differentiates two clusters (Iberian Peninsula and Canary Islands). The sequences included are, apart from the reference strains, all the Spanish EV13 (61 isolates and 3 sequences obtained directly from cerebrospinal fluid) and two American isolates. Model of nucleotide substitution: Kimura two parameters. Phylogenetic tree reconstructed with the neighbor joining method, and bootstrap analysis with 1,000 pseudoreplicate datasets.