Figure 3. Analytical isoelectric focusing (IEF) of serially diluted broth induced enzymes. The isoelectric points (pIs) of the two prominent bands midway and at the bottom of the gel are 6.9 and >9.0. Lanes 1–3: uninduced enzyme serially diluted 1:1, 1:2, and 1:4. Lanes 4–6: enzyme induced with 8 μg/mL of cefoxitin serially diluted 1:1, 1:2, and 1:4. Lanes 7–9: enzyme induced with 4 μg/mL of imipenem serially diluted 1:1, 1:2 and 1:4. Lane 10: NOR-1 control, induced with 16 μg/mL of cefoxitin.
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