Emerging Infectious Disease ISSN: 1080-6059

NmcA Carbapenem-hydrolyzing Enzyme in Enterobacter cloacae in North America¹

Sudha Pottumarthy*, Ellen Smith Moland†, Stefan Juretschko*, Susan R. Swanzy*, Kenneth S. Thomson†, and Thomas R. Fritsche*

Author affiliations: *University of Washington School of Medicine, Seattle, Washington, USA; †Creighton University Medical Center, Omaha, Nebraska, USA

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Figure 3

Analytical isoelectric focusing (IEF) of serially diluted broth induced enzymes. The isoelectric points (pIs) of the two prominent bands midway and at the bottom of the gel are 6.9 and >9.0. Lanes 1–3: uninduced enzyme serially diluted 1:1, 1:2, and 1:4. Lanes 4–6: enzyme induced with 8 μg/mL of cefoxitin serially diluted 1:1, 1:2, and 1:4. Lanes 7–9: enzyme induced with 4 μg/mL of imipenem serially diluted 1:1, 1:2 and 1:4. Lane 10: NOR-1 control, induced with 16 μg/mL of cefoxitin.

Figure 3. Analytical isoelectric focusing (IEF) of serially diluted broth induced enzymes. The isoelectric points (pIs) of the two prominent bands midway and at the bottom of the gel are 6.9 and >9.0. Lanes 1–3: uninduced enzyme serially diluted 1:1, 1:2, and 1:4. Lanes 4–6: enzyme induced with 8 μg/mL of cefoxitin serially diluted 1:1, 1:2, and 1:4. Lanes 7–9: enzyme induced with 4 μg/mL of imipenem serially diluted 1:1, 1:2 and 1:4. Lane 10: NOR-1 control, induced with 16 μg/mL of cefoxitin.

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¹This work was presented in part at the 13th Annual European Congress of Clinical Microbiology and Infectious Diseases, Glasgow, Scotland, 2003.

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