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Volume 14, Number 9—September 2008
Research

Pediatric Parapneumonic Empyema, Spain

Ignacio ObandoComments to Author , Carmen Muñoz-Almagro, Luis A. Arroyo, David Tarrago, David Sanchez-Tatay, David Moreno-Perez, Sahar S. Dhillon, Cristina Esteva, Susanna Hernandez-Bou, Juan J. Garcia-Garcia, William P. Hausdorff, and Angela B. Brueggemann
Author affiliations: Virgen del Rocio Children’s Hospital, Seville, Spain (I. Obando, L.A. Arroyo, D. Sanchez-Tatay); Sant Joan de Deu Hospital, Barcelona, Spain (C. Muñoz-Almagro, C. Esteva, S. Hernandez-Bou, J.J. Garcia-Garcia); Spanish Reference Laboratory for Pneumococci, Madrid, Spain (D. Tarrago); Carlos de Haya Children’s Hospital, Malaga, Spain (D. Moreno-Perez); University of Oxford, Oxford, UK (S.S. Dhillon, A.B. Brueggemann);; GlaxoSmithKline Biologicals, Rixensart, Belgium (W.P. Hausdorff);

Main Article

Table 3

Sequence types and serotypes among 81 pneumococci detected in pleural fluid

Serotype* Total Sequence type (n)
1 43 306and SLVs (23)† , 228 (11)‡, 304and SLVs (8)†§, 2373 (1)
5 9 289and SLV (9)†
3 8 180 (5), 260 (2), 2590 (1)§
14 7 156 (6), 17 (1)§
19A 6 276 (2), 81 (1)§, 202 (1)§, 
1201 (1)‡, 2013 (1)§
7F 4 191 (4)
6A 2 135 (1)‡, 2377 (1)§
9V 2 838 (2)§

*Included are 8 strains that were culture and PCR negative, or not serotyped, but whose full genotyping by multilocus sequence typing (www.mlst.net) enabled serotypes 1, 5, and 7F to be predicted. These serotypes were predicted because in each case the sequence type (ST) was identical or closely related to a known genotype for serotypes 1, 5 or 7F that have never been identified with anything other than those respective serotypes. These included ST (no.): 306 (2), 228 (1), 2373 (1), 2378 (1), 2561 (1), 1223 (1), and 191 (1).
†SLV, single locus variant (i.e., differs at only 1 MLST locus and thus is a closely related genotype). Major serotypes 1 and 5 ST groups included (no. strains): ST306 (18) and SLVs 2375, 2376, 2378, and 2561 (1 each); ST304 (5) and SLVs 2374 (2), 2371 (1); ST289 (5), and SLV 1223 (4).
‡Recovered only in Sevilla/Malaga.
§Recovered only in Barcelona.

Main Article

Page created: July 13, 2010
Page updated: July 13, 2010
Page reviewed: July 13, 2010
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