Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 22, Number 5—May 2016
Research

Expansion of Shiga Toxin–Producing Escherichia coli by Use of Bovine Antibiotic Growth Promoters

Jong-Chul Kim, Linda Chui, Yang Wang, Jianzhong Shen, and Byeonghwa JeonComments to Author 
Author affiliations: University of Alberta, Edmonton, Alberta, Canada (J.-C. Kim, L. Chui, B. Jeon); Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety at China Agricultural University, Beijing, China (Y. Wang, J. Shen)

Main Article

Figure 1

Induction of Shiga toxin (Stx)2 phage propagation and SOS response by bovine antibiotic growth promoters (bAGPs). A) Stx2 phage induction in Escherichia coli O157:H7 EDL933 after 3 h exposure to subtherapeutic concentrations of bAGPs, including monensin (MON), tylosin (TYL), chlortetracycline (CTC), and oxytetracycline (OTC). Ciprofloxacin (CIP) was a control for phage induction. E. coli C600 was used as a phage-susceptible strain. B) Induction of stx2 expression by bAGPs. Fluorescence from an s

Figure 1. Induction of Shiga toxin (Stx)2 phage propagation and SOS response by bovine antibiotic growth promoters (bAGPs). A) Stx2 phage induction in Escherichia coli O157:H7 EDL933 after 3 h exposure to subtherapeutic concentrations of bAGPs, including monensin (MON), tylosin (TYL), chlortetracycline (CTC), and oxytetracycline (OTC). Ciprofloxacin (CIP) was a control for phage induction. E. coli C600 was used as a phage-susceptible strain. B) Induction of stx2 expression by bAGPs. Fluorescence from an stx2::gfp transcriptional fusion indicates the level of Stx2 phage induction. C) Induction of recA transcription by bAGPs. The level of recA expression indicates the level of SOS response induction. The results show means and SDs of 3 independent experiments. Statistical significance was analyzed by using the Student t-test. **p<0.01, ***p<0.001, ****p<0.0001.

Main Article

Page created: April 13, 2016
Page updated: April 13, 2016
Page reviewed: April 13, 2016
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external