Figure 3. Histologic section through the anterior gastric chamber of a moribund juvenile Litopenaeusvannamei that was injected with an inoculum prepared with tissue cell culture media from BGMK cells exposed to Taura syndrome virus (TSV) (day 7 postexposure). A) The arrows point to a portion of cuticular epithelium displaying diagnostic acute-phase TSV lesions (hematoxylin/eosin-phloxin stain; 50x). B) The dashed arrows point to a portion of the stomach epithelium from the same shrimp, where digoxigenin (DIG)-labeled TSV-specific gene probes were reacted by in situ hybridization (ISH), resulting in the deposition of a black precipitate on areas where the probe hybridized with target TSV (Bismarck Brown counterstain; 50x).
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